| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DBX2 |
| Uniprot No | Q6ZNG2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-339aa |
| 氨基酸序列 | MLPSAVAAHA GAYWDVVASS ALLNLPAAPG FGNLGKSFLI ENLLRVGGAP TPRLQPPAPH DPATALATAG AQLRPLPASP VPLKLCPAAE QVSPAGAPYG TRWAFQVLSP SADSARLPGR APGDRDCTFQ PSAPAPSKPF LLSTPPFYSA CCGGSCRRPA SSTAFPREES MLPLLTQDSN SKARRGILRR AVFSEDQRKA LEKMFQKQKY ISKTDRKKLA INLGLKESQV KIWFQNRRMK WRNSKEKEVL SNRCIQEVGL QEDPLSRSAL GFPSPCPSIW DVPQQHSSPR WRENSPEPSE RLIQESSGAP PPEANSLQGA LYLCSEEEAG SKGVLTGAV |
| 分子量 | 63.69 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DBX2蛋白的模拟参考文献示例(注：文献信息为假设性描述，仅供格式参考)：
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1. **文献名称**: *Functional analysis of recombinant human DBX2 in neural progenitor differentiation*
**作者**: Smith A, et al.
**摘要**: 研究利用大肠杆菌表达系统制备重组人DBX2蛋白，并验证其促进胚胎干细胞向脊髓中间神经元分化的功能，证实DBX2通过调控特定靶基因(如Nkx6.1)发挥作用。
2. **文献名称**: *Purification and structural characterization of DBX2 homeodomain protein*
**作者**: Chen L, et al.
**摘要**: 报道通过HEK293细胞表达重组人DBX2蛋白的优化方法，经亲和层析纯化后，利用X射线晶体学解析其DNA结合结构域的三维结构，揭示其与特定DNA序列互作的关键位点。
3. **文献名称**: *DBX2 regulates midbrain dopaminergic neuron development via SHH signaling*
**作者**: Tanaka K, et al.
**摘要**: 通过体外重组DBX2蛋白处理胚胎小鼠中脑细胞，发现其通过增强Sonic Hedgehog(SHH)通路活性，调控多巴胺能神经元的迁移和存活。
4. **文献名称**: *In vitro screening of DBX2-binding partners using recombinant protein pull-down assays*
**作者**: Wang Y, et al.
**摘要**: 利用重组人DBX2蛋白进行Pull-down实验，筛选出与其相互作用的蛋白质(如Pbx1和Lmx1b)，为理解DBX2在神经元分化和轴突导向中的分子机制提供依据。
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如需真实文献，建议通过PubMed或Google Scholar检索关键词“recombinant human DBX2”或“DBX2 protein function”。
Recombinant human DBX2 protein is derived from the DBX2 (Developmental Brain Homeobox 2) gene, a member of the homeobox transcription factor family characterized by a conserved DNA-binding homeodomain. DBX2 plays critical roles in embryonic development, particularly in the regulation of neural progenitor cell differentiation and the patterning of the central nervous system. During neurogenesis, it is involved in establishing boundaries between distinct neuronal populations and guiding dorsoventral patterning of the spinal cord. DBX2 functions as a transcriptional repressor, interacting with specific DNA sequences to regulate downstream genes essential for cell fate determination and tissue morphogenesis.
The recombinant form is typically produced using expression systems like E. coli or mammalian cells, ensuring high purity and bioactivity for experimental use. Researchers utilize recombinant DBX2 to study its molecular mechanisms in neurodevelopment, its interactions with co-factors like other transcription regulators, and its potential role in diseases. Emerging evidence suggests DBX2 dysregulation may contribute to neurodevelopmental disorders or certain cancers, as homeobox proteins are increasingly linked to oncogenesis. Current applications span in vitro assays, gene editing studies, and disease modeling, providing insights into cellular differentiation pathways and therapeutic targeting strategies.
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