| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DBX1 |
| Uniprot No | A6NMT0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-382aa |
| 氨基酸序列 | MMFPGLLAPPAGYPSLLRPTPTLTLPQSLQSAFSGHSSFLVEDLIRISRPPAYLPRSVPTASMSPPRQGAPTALTDTGASDLGSPGPGSRRGGSPPTAFSPASETTFLKFGVNAILSSGPRTETSPALLQSVPPKTFAFPYFEGSFQPFIRSSYFPASSSVVPIPGTFSWPLAARGKPRRGMLRRAVFSDVQRKALEKMFQKQKYISKPDRKKLAAKLGLKDSQDTARRTEPAPDSTPRPRASPGAPDLTSASPFFSPAGTFQVKIWFQNRRMKWRNSKERELLSSGGCREQTLPTKLNPHPDLSDVGQKGPGNEEEEEGPGSPSHRLAYHASSDPQHLRDPRLPGPLPPSPAHSSSPGKPSDFSDSEEEEEGEEQEEITVS |
| 分子量 | 42.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与重组人DBX1蛋白相关的3篇参考文献示例(基于公开研究主题的合理推断，非真实文献)：
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1. **文献名称**：*"Functional Characterization of Recombinant Human DBX1 in Neural Progenitor Cell Differentiation"*
**作者**：Smith J, et al.
**摘要**：本研究成功在大肠杆菌中表达并纯化重组人DBX1蛋白，证明其在体外能够促进神经祖细胞向特定中间神经元亚型分化，提示DBX1在神经发育中的关键作用。
2. **文献名称**：*"Structural and Biochemical Analysis of DBX1 Reveals Its DNA-Binding Specificity"*
**作者**：Garcia R, et al.
**摘要**：通过重组人DBX1蛋白的晶体结构解析，发现其C端同源结构域能够特异性结合富含AT序列的DNA区域，为解释DBX1在基因转录调控中的机制提供了结构基础。
3. **文献名称**：*"DBX1 Regulates Spinal Cord Development via Interactions with Hedgehog Signaling Components"*
**作者**：Chen L, et al.
**摘要**：利用哺乳动物细胞表达系统制备重组DBX1蛋白，结合体内实验验证其通过调控Hedgehog信号通路参与脊髓神经元的模式形成和轴突导向。
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**注**：以上为根据研究领域推断的示例文献，实际引用时需以真实发表的论文为准。建议通过PubMed、Google Scholar等平台以“recombinant human DBX1 protein”或“DBX1 function”为关键词检索最新研究成果。
Recombinant human DBX1 protein is a genetically engineered form of the DBX1 (Developing Brain Homeobox 1) transcription factor, which plays a critical role in neural development. DBX1 contains a conserved homeodomain that enables sequence-specific DNA binding, regulating the expression of downstream genes involved in neuronal subtype specification. It is particularly essential for the differentiation of spinal cord V0 interneurons, which contribute to locomotor circuits by modulating motor coordination.
Studies suggest DBX1 acts as a fate determinant by repressing specific transcription factors (e.g., Evx1) while activating others, thereby directing progenitor cells toward inhibitory neuronal lineages. Its expression is tightly regulated during embryogenesis, with disruptions linked to neurodevelopmental anomalies or neurological disorders. Recombinant DBX1 protein, typically produced in E. coli or mammalian expression systems, retains functional DNA-binding activity and is widely used to investigate molecular mechanisms underlying neural patterning.
Researchers employ this protein in in vitro assays (e.g., EMSA, reporter gene studies) to map DNA interaction motifs, analyze transcriptional regulatory networks, or model cell differentiation pathways. Additionally, it serves as a tool to study DBX1’s potential role in neurodegenerative diseases or cancer, where homeobox genes are often dysregulated. Structural studies using recombinant DBX1 also aid in designing therapeutic molecules targeting DBX1-associated pathways.
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