| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DAZAP2 |
| Uniprot No | Q15038 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-168aa |
| 氨基酸序列 | MNSKGQYPTQPTYPVQPPGNPVYPQTLHLPQAPPYTDAPPAYSELYRPSFVHPGAATVPTMSAAFPGASLYLPMAQSVAVGPLGSTIPMAYYPVGPIYPPGSTVLVEGGYDAGARFGAGATAGNIPPPPPGCPPNAAQLAVMQGANVLVTQRKGNFFMGGSDGGYTIW |
| 分子量 | 43.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是几条与重组人DAZAP2蛋白相关的参考文献示例(虚构内容，仅作格式参考)：
---
1. **文献名称**: *Expression and Functional Analysis of Recombinant Human DAZAP2 in Mammalian Cells*
**作者**: Liu Y, et al.
**摘要**: 研究报道了在HEK293细胞中高效表达重组人DAZAP2蛋白的优化方法，并验证其通过调控mRNA稳定性参与细胞增殖的功能。
2. **文献名称**: *DAZAP2 Modulates Alternative Splicing by Interaction with RNA-Binding Proteins*
**作者**: Smith J, et al.
**摘要**: 通过免疫共沉淀和质谱分析发现，重组DAZAP2蛋白能与SRSF1和hnRNPA1相互作用，影响神经细胞中靶基因的可变剪接。
3. **文献名称**: *Crystal Structure of Human DAZAP2 Reveals a Novel RNA Recognition Motif*
**作者**: Chen L, et al.
**摘要**: 首次解析了重组人DAZAP2蛋白的晶体结构，揭示其非典型RNA结合域，为研究其在精子发生中的分子机制提供结构基础。
4. **文献名称**: *DAZAP2 Deficiency Disrupts Embryonic Development via mRNA Translation Defects*
**作者**: Wang H, et al.
**摘要**: 利用重组DAZAP2蛋白挽救实验，证实其在胚胎早期发育中通过调控特定mRNA的翻译效率维持细胞正常分化。
---
**备注**：以上文献信息为模拟生成，实际研究中请通过PubMed或Web of Science等平台检索真实文献。
**Background of Recombinant Human DAZAP2 Protein**
DAZAP2 (Deleted in Azoospermia Associated Protein 2) is a ubiquitously expressed RNA-binding protein involved in diverse cellular processes, including RNA metabolism, transport, and translational regulation. Initially identified through its interaction with DAZ (Deleted in Azoospermia) proteins, DAZAP2 plays roles in gametogenesis, particularly spermatogenesis, though its functions extend beyond reproductive biology. Structurally, it contains an N-terminal RNA-recognition motif (RRM) and a proline-rich C-terminal region, facilitating interactions with RNA, proteins, and components of cellular signaling pathways.
Recombinant human DAZAP2 protein is engineered using molecular cloning techniques, often expressed in bacterial or eukaryotic systems for biochemical and functional studies. It serves as a critical tool to investigate DAZAP2’s role in development, disease, and cellular stress responses. Research links DAZAP2 to cancer, where it may act as a tumor suppressor or promoter depending on context, and neurodevelopmental disorders, with studies suggesting involvement in mRNA stability and splicing anomalies. Its dysregulation has also been associated with neurodegenerative diseases. The recombinant protein enables exploration of DAZAP2’s molecular mechanisms, offering potential therapeutic insights for related pathologies.
×
