| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DAZ4 |
| Uniprot No | Q86SG3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-390aa |
| 氨基酸序列 | MSAANPETPNSTISREASTQSSSAAASQGWVLPEGKIVPNTVFVGGIDARMDETEIGSCFGRYGSVKEVKIITNRTGVSKGYGFVSFVNDVDVQKIVGSQIHFHGKKLKLGPAIRKQKLCARHVQPRPLVVNPPPPPQFQNVWRNPNTETYLQPQITPNPVTQHVQAYSAYPHSPGQVITGCQLLVYNYQEYPTYPDSAFQVTTGYQLPVYNYQPFPAYPRSPFQVTAGYQLPVYNYQAFPAYPNSPFQVATGYQFPVYNYQPFPAYPSSPFQVTAGYQLPVYNYQAFPAYPNSPFQVATGYQFPVYNYQAFPAYPNSPVQVTTGYQLPVYNYQAFPAYPNSAVQVTTGYQFHVYNYQMPPQCPVGEQRRNLWTEAYKWWYLVCLIQRRD |
| 分子量 | 70.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DAZ4蛋白的参考文献示例，内容基于领域内相关研究综合概括：
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1. **文献名称**：*Expression and functional characterization of recombinant human DAZ4 protein in Escherichia coli*
**作者**：Venables, J.P., et al.
**摘要**：本研究成功将人DAZ4基因克隆至大肠杆菌表达系统，并通过亲和层析纯化获得高纯度重组蛋白。实验验证了DAZ4蛋白在体外能够特异性结合靶RNA序列，暗示其在精子发生中可能通过RNA调控发挥作用。
2. **文献名称**：*DAZ4 protein interacts with germ cell-specific proteins in human testis*
**作者**：Reijo, R., et al.
**摘要**：通过免疫共沉淀技术，发现重组表达的DAZ4蛋白与人类睾丸组织中多种生殖细胞蛋白(如BOLL、PUM2)存在相互作用，提示其可能在精子形成过程中参与多蛋白复合体的组装与功能调控。
3. **文献名称**：*DAZ family proteins: Structural analysis of DAZ4 reveals conserved RRM motifs critical for fertility*
**作者**：Ruggiu, M., et al.
**摘要**：作者解析了重组人DAZ4蛋白的晶体结构，发现其RNA识别基序(RRM)的保守性。体外功能实验表明，突变关键氨基酸残基会显著降低DAZ4与配体RNA的结合能力，证明结构-功能关系对男性生育力的重要性。
4. **文献名称**：*Recombinant DAZ4 as a potential biomarker for male infertility screening*
**作者**：Yang, X., et al.
**摘要**：通过比较不育男性与健康对照组血清样本，发现重组DAZ4蛋白抗体阳性率与无精症显著相关。研究表明，DAZ4可能作为分子标记物用于临床无创诊断精子发生障碍。
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**说明**：以上文献名称与作者为模拟归纳性示例，实际研究中需通过数据库(如PubMed)以“DAZ4”、“deleted in azoospermia”、“recombinant protein”等关键词检索具体文献。DAZ家族蛋白的核心研究多集中于其RNA结合功能、生殖细胞特异性表达及与男性不育的关联。
The human DAZ (Deleted in Azoospermia) protein family, encoded by genes on the Y chromosome, plays a critical role in spermatogenesis. DAZ4. one of the four major DAZ paralogs, is specifically implicated in germ cell development and meiosis. Its absence or mutation is linked to male infertility, particularly azoospermia or severe oligospermia. DAZ proteins contain conserved RNA-binding motifs (RRM and DAZ motifs), enabling interactions with mRNAs involved in gametogenesis. Recombinant DAZ4 protein, produced via expression systems like *E. coli* or mammalian cells, serves as a vital tool for studying molecular mechanisms underlying fertility. Researchers employ it to analyze RNA-binding specificity, protein-protein interactions, and regulatory pathways in germ cells. Its recombinant form also facilitates the development of diagnostic assays or therapeutic strategies for infertility. Due to the DAZ family’s structural redundancy and complexity, isolating DAZ4-specific functions remains challenging, highlighting the need for targeted recombinant protein studies. Current applications extend to modeling DAZ4 dysfunction in vitro and exploring its role in pluripotent stem cell differentiation. This protein’s study bridges gaps in understanding post-transcriptional regulation during spermatogenesis and Y chromosome-linked infertility.
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