| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DBF4 |
| Uniprot No | Q8NFT6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-674aa |
| 氨基酸序列 | MNSGAMRIHSKGHFQGGIQVKNEKNRPSLKSLKTDNRPEKSKCKPLWGKVFYLDLPSVTISEKLQKDIKDLGGRVEEFLSKDISYLISNKKEAKFAQTLGRISPVPSPESAYTAETTSPHPSHDGSSFKSPDTVCLSRGKLLVEKAIKDHDFIPSNSILSNALSWGVKILHIDDIRYYIEQKKKELYLLKKSSTSVRDGGKRVGSGAQKTRTGRLKKPFVKVEDMSQLYRPFYLQLTNMPFINYSIQKPCSPFDVDKPSSMQKQTQVKLRIQTDGDKYGGTSIQLQLKEKKKKGYCECCLQKYEDLETHLLSEQHRNFAQSNQYQVVDDIVSKLVFDFVEYEKDTPKKKRIKYSVGSLSPVSASVLKKTEQKEKVELQHISQKDCQEDDTTVKEQNFLYKETQETEKKLLFISEPIPHPSNELRGLNEKMSNKCSMLSTAEDDIRQNFTQLPLHKNKQECILDISEHTLSENDLEELRVDHYKCNIQASVHVSDFSTDNSGSQPKQKSDTVLFPAKDLKEKDLHSIFTHDSGLITINSSQEHLTVQAKAPFHTPPEEPNECDFKNMGSLPSGKIHRKVKIILGRNRKENLEPNAEFDKRTEFITQEEDRICSSPVQSLLDLFQTSEEKSEFLGFTSYTEKSGICNVLDIWEEENSDNLLTAFFSSPSTSTFTGF |
| 分子量 | 99.66 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DBF4蛋白的3篇参考文献示例(文献信息为虚拟构建，供参考)：
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1. **文献名称**：*Structural and Functional Analysis of Recombinant Human DBF4 in CDC7 Kinase Activation*
**作者**：Chen L. et al.
**摘要**：本研究成功表达并纯化了重组人DBF4蛋白，证实其通过结合CDC7激酶调控DNA复制起始。通过体外激酶实验，揭示了DBF4的N端结构域对CDC7激活的关键作用，为靶向该复合物的抗癌药物设计提供依据。
2. **文献名称**：*DBF4 Overexpression Promotes Genomic Instability in Cancer Cells*
**作者**：Watanabe K. et al.
**摘要**：探讨了重组DBF4蛋白过表达对肿瘤细胞周期的影响，发现其异常表达导致DNA复制应激和染色体错误，提示DBF4可能作为癌症治疗中基因组稳定性的调控靶点。
3. **文献名称**：*Development of a High-Yield Expression System for Recombinant Human DBF4 in E. coli*
**作者**：Zhang Y. et al.
**摘要**：优化了大肠杆菌中重组人DBF4蛋白的可溶性表达方案，利用亲和层析和分子筛纯化获得高纯度蛋白，为后续功能研究与药物筛选建立了技术基础。
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**说明**：上述文献为虚拟示例，实际研究中建议通过PubMed或Web of Science检索关键词“recombinant human DBF4”或“DBF4 CDC7 complex”获取真实文献。
Recombinant human DBF4 protein is a key regulatory factor involved in the initiation of DNA replication during the cell cycle. DBF4. also known as ASK (Activator of S Phase Kinase), functions as an essential co-activator of the CDC7 kinase, forming the CDC7-DBF4 complex. This complex phosphorylates components of the minichromosome maintenance (MCM) helicase, enabling the activation of replication origins during the G1/S phase transition. Dysregulation of DBF4 has been linked to genomic instability, replication stress, and tumorigenesis, highlighting its critical role in cell cycle progression and genome integrity.
Recombinant DBF4 is typically produced using heterologous expression systems (e.g., E. coli or mammalian cells) with affinity tags (e.g., His-tag) for purification. It serves as a vital tool for studying DNA replication mechanisms, cell cycle checkpoints, and cancer biology. Researchers use it to investigate how CDC7-DBF4 activity is regulated by cyclin-dependent kinases (CDKs) or stress responses, as well as to screen inhibitors targeting replication-initiation pathways for anticancer therapies. Its recombinant form enables precise biochemical assays, structural studies, and functional analyses in vitro or in cell-based models, bridging molecular insights with therapeutic applications.
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