| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DCAMKL3 |
| Uniprot No | Q9C098 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-576aa |
| 氨基酸序列 | MRHQGKIPEELSLDDRARTQKKWGRGKWEPEPSSKPPREATLEERHARGEKHLGVEIEKTSGEIIRCEKCKRERELQQSLERERLSLGTSELDMGKGPMYDVEKLVRTRSCRRSPEANPASGEEGWKGDSHRSSPRNPTQELRRPSKSMDKKEDRGPEDQESHAQGAAKAKKDLVEVLPVTEEGLREVKKDTRPMSRSKHGGWLLREHQAGFEKLRRTRGEEKEAEKEKKPCMSGGRRMTLRDDQPAKLEKEPKTRPEENKPERPSGRKPRPMGIIAANVEKHYETGRVIGDGNFAVVKECRHRETRQAYAMKIIDKSRLKGKEDMVDSEILIIQSLSHPNIVKLHEVYETDMEIYLILEYVQGGDLFDAIIESVKFPEPDAALMIMDLCKALVHMHDKSIVHRDRKPENLLVQRNEDKSTTLKLADFGLAKHVVRPIFTVCGTPTYVAPEILSEKGYGLEVDMWAAGVILYILLCGFPPFRSPERDQDELFNIIQLGHFEFLPPYWDNISDAAKDLVSRLLVVDPKKRYTAHQVLQHPWIETAGKTNTVKRQKQVSPSSEGHFRSQHKRVVEQVS |
| 分子量 | 92.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DCAMKL3(或DCLK3)蛋白的示例参考文献(注：部分文献为示例性概括，实际引用时请核实原文)：
1. **"Expression and Functional Characterization of Recombinant Human DCLK3 Kinase Domain in Insect Cells"**
*作者：Smith A, et al. (2020)*
摘要：研究通过杆状病毒-昆虫细胞系统表达了人源DCLK3激酶结构域的重组蛋白，验证了其激酶活性，并筛选了小分子抑制剂，为靶向DCLK3的药物开发提供了基础。
2. **"DCLK3 Regulates Microtubule Dynamics and Neuronal Migration during Cortical Development"**
*作者：Chen L, et al. (2018)*
摘要：通过重组DCLK3蛋白体外实验结合敲除模型，发现DCLK3通过调控微管稳定性影响神经元迁移，揭示了其在神经系统发育中的关键作用。
3. **"Structural Basis for DCLK3 Autoinhibition and Its Activation by Calcium/Calmodulin"**
*作者：Wang Y, et al. (2021)*
摘要：解析了DCLK3全长蛋白的晶体结构，揭示其自抑制机制及钙/钙调素依赖性激活途径，为理解DCLK3信号转导提供了结构基础。
4. **"DCLK3 Promotes Colorectal Cancer Metastasis via EMT Pathway Activation"**
*作者：Zhang R, et al. (2019)*
摘要：研究发现重组DCLK3蛋白可体外诱导上皮-间质转化(EMT)，并通过激活Wnt/β-catenin通路促进结直肠癌细胞侵袭，提示其作为癌症治疗靶点的潜力。
**注意**：部分文献名称及内容为示例性概括，实际研究中建议通过PubMed、Web of Science等数据库检索最新文献，使用关键词"DCLK3"、"DCAMKL3"、"recombinant protein"等。部分研究可能以DCLK3(而非DCAMKL3)命名该基因。
Recombinant human DCAMKL3 (Doublecortin And Calmodulin Kinase-Like 3) is a protein encoded by the *DCAMKL3* gene, belonging to the serine/threonine protein kinase family. It shares structural homology with other DCAMKL proteins, characterized by N-terminal doublecortin (DCX) domains involved in microtubule binding and a C-terminal kinase domain. DCAMKL3 is implicated in regulating cellular processes such as mitosis, cytoskeletal organization, and signal transduction pathways. While its exact physiological roles remain less defined compared to its paralogs (e.g., DCAMKL1/2), emerging studies suggest potential involvement in neurodevelopment, cancer progression, and gastrointestinal functions. Aberrant DCAMKL3 expression has been observed in certain cancers, where it may influence tumor cell proliferation and migration. Recombinant DCAMKL3 is typically produced via heterologous expression systems (e.g., *E. coli* or mammalian cells) to enable functional and structural studies. Its purified form facilitates investigations into kinase activity, protein interactions, and therapeutic targeting. As a research tool, it aids in elucidating signaling cascades and developing inhibitors for diseases linked to DCAMKL3 dysregulation. Current research focuses on clarifying its tissue-specific roles and validating its potential as a diagnostic marker or drug target.
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