| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DCAF12L1 |
| Uniprot No | Q5VU92 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-463aa |
| 氨基酸序列 | MAQQQTGSRKRKAPAVEAGAESSPSQGLAAADGEGPLLLKRQRRPATYRSMAHYLKVREVGGWGPARLQGFDGELRGYAVQRLPELLTERQLELGTVNKVFASQWLNSRQVVCGTKCNTLFVVDVESGHIARIPLLRDSEARLAQDQQGCGIHAIELNPSKTLLATGGENPNSLAIYQLPSLDPLCLGDRHGHKDWIFAVAWLSDTVAVSGSRDGTVALWRMDPDKFDDTVAWHSEVGLPVYAHIRPRDVEAIPRAIINPSNRKVRALACGGKNQELGAVSLDGYFHLWKAGSALSRLLSIRLPYFRDNVCLTYCDDMSVYAVGSHSHVSFLDLRQDQQNIRPLCSREGGTGVRSLSFYRHIITVGTGQGSLLFYDVRAQKFLEERASATLESSSGPARRKLRLACGRGWLNHNDFWVNYFGGMEVFPNALYTHCYNWPEMKLFVAGGPLPAGLHGNYAGLWS |
| 分子量 | 51 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DCAF12L1蛋白的参考文献举例(内容为虚构示例，实际文献需通过学术数据库验证)：
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1. **"Functional characterization of DCAF12L1 in ubiquitin-proteasome system regulation"**
*作者：Smith A, et al. (2021)*
**摘要**：研究通过重组表达人DCAF12L1蛋白，证实其作为CUL4-DDB1 E3连接酶复合物的底物适配器，参与特定蛋白底物的泛素化降解，可能调控细胞周期进程。
2. **"Structural insights into DCAF12L1 and its interaction with viral proteins"**
*作者：Li J, et al. (2019)*
**摘要**：利用重组DCAF12L1蛋白解析其晶体结构，揭示其WD40结构域介导与HIV-1 Vif蛋白的相互作用，为抗病毒靶点开发提供依据。
3. **"DCAF12L1 expression and purification: A protocol for high-yield recombinant protein production"**
*作者：Chen R, et al. (2020)*
**摘要**：优化了人DCAF12L1在大肠杆菌中的重组表达和纯化流程，获得高纯度蛋白用于后续生化及功能研究。
4. **"DCAF12L1 knockdown impairs DNA damage response via destabilizing BRCA1"**
*作者：Wang Y, et al. (2022)*
**摘要**：通过重组DCAF12L1蛋白的体外实验，证明其调控BRCA1稳定性，缺失会导致DNA修复缺陷，提示其在癌症中的潜在作用。
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**注意**：以上文献为示例，实际研究中相关论文可能较少，建议通过 **PubMed/Google Scholar** 检索关键词 "DCAF12L1 recombinant" 或联系领域综述获取具体文献。DCAF家族蛋白的研究较多集中在其他成员(如DCAF1/DYRK2)，可拓展相关关键词。
DCAF12L1 (DDB1- and CUL4-associated factor 12-like 1) is a poorly characterized protein belonging to the WD40 repeat-containing DCAF family, which functions as substrate receptors for CUL4-DDB1 E3 ubiquitin ligase complexes. These complexes mediate substrate-specific ubiquitination, targeting proteins for proteasomal degradation. DCAF12L1 shares structural homology with DCAF12. featuring multiple WD40 repeats that facilitate protein-protein interactions. While its exact physiological role remains unclear, studies suggest potential involvement in cellular processes such as DNA repair, cell cycle regulation, or apoptosis. Dysregulation of DCAF family proteins is frequently implicated in cancer, making DCAF12L1 a subject of emerging interest in oncogenesis research.
Recombinant human DCAF12L1 protein is produced using heterologous expression systems (e.g., E. coli or mammalian cells) for functional studies. Its recombinant form enables biochemical characterization, including binding partner identification, structural analysis, and enzymatic activity assays. Researchers utilize this tool to investigate its interaction with CUL4-DDB1 complexes, substrate recognition mechanisms, and potential roles in ubiquitination pathways. Current challenges include elucidating tissue-specific expression patterns and validating physiological substrates. As an understudied member of the ubiquitin-proteasome system, understanding DCAF12L1 may reveal novel therapeutic targets for diseases involving protein homeostasis disorders, particularly in oncology.
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