| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DDB1 |
| Uniprot No | Q9NZJ0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1140aa |
| 氨基酸序列 | MSYNYVVTAQKPTAVNGCVTGHFTSAEDLNLLIAKNTRLEIYVVTAEGLRPVKEVGMYGKIAVMELFRPKGESKDLLFILTAKYNACILEYKQSGESIDIITRAHGNVQDRIGRPSETGIIGIIDPECRMIGLRLYDGLFKVIPLDRDNKELKAFNIRLEELHVIDVKFLYGCQAPTICFVYQDPQGRHVKTYEVSLREKEFNKGPWKQENVEAEASMVIAVPEPFGGAIIIGQESITYHNGDKYLAIAPPIIKQSTIVCHNRVDPNGSRYLLGDMEGRLFMLLLEKEEQMDGTVTLKDLRVELLGETSIAECLTYLDNGVVFVGSRLGDSQLVKLNVDSNEQGSYVVAMETFTNLGPIVDMCVVDLERQGQGQLVTCSGAFKEGSLRIIRNGIGIHEHASIDLPGIKGLWPLRSDPNRETDDTLVLSFVGQTRVLMLNGEEVEETELMGFVDDQQTFFCGNVAHQQLIQITSASVRLVSQEPKALVSEWKEPQAKNISVASCNSSQVVVAVGRALYYLQIHPQELRQISHTEMEHEVACLDITPLGDSNGLSPLCAIGLWTDISARILKLPSFELLHKEMLGGEIIPRSILMTTFESSHYLLCALGDGALFYFGLNIETGLLSDRKKVTLGTQPTVLRTFRSLSTTNVFACSDRPTVIYSSNHKLVFSNVNLKEVNYMCPLNSDGYPDSLALANNSTLTIGTIDEIQKLHIRTVPLYESPRKICYQEVSQCFGVLSSRIEVQDTSGGTTALRPSASTQALSSSVSSSKLFSSSTAPHETSFGEEVEVHNLLIIDQHTFEVLHAHQFLQNEYALSLVSCKLGKDPNTYFIVGTAMVYPEEAEPKQGRIVVFQYSDGKLQTVAEKEVKGAVYSMVEFNGKLLASINSTVRLYEWTTEKELRTECNHYNNIMALYLKTKGDFILVGDLMRSVLLLAYKPMEGNFEEIARDFNPNWMSAVEILDDDNFLGAENAFNLFVCQKDSAATTDEERQHLQEVGLFHLGEFVNVFCHGSLVMQNLGETSTPTQGSVLFGTVNGMIGLVTSLSESWYNLLLDMQNRLNKVIKSVGKIEHSFWRSFHTERKTEPATGFIDGDLIESFLDISRPKMQEVVANLQYDDGSGMKREATADDLIKVVEELTRIH |
| 分子量 | 153.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DDB1蛋白的3篇参考文献，涵盖结构、功能及疾病关联研究：
1. **文献名称**：*"Structure of the DDB1-CUL4A Complex and Recognition of UV-Damaged DNA"*
**作者**：Angers S. et al.
**摘要**：解析了DDB1与CUL4A蛋白的复合物晶体结构，揭示了其作为泛素连接酶复合物的组成，并阐明其在紫外线损伤DNA识别中的关键作用。
2. **文献名称**：*"Structural Basis for DNA Damage Recognition by the DDB1-DDB2 Complex"*
**作者**：Scrima A. et al.
**摘要**：通过X射线晶体学分析，阐明DDB1-DDB2异源二聚体如何特异性识别紫外线诱导的DNA损伤(如嘧啶二聚体)，并启动后续核苷酸切除修复通路。
3. **文献名称**：*"Hepatitis C Virus Non-structural Protein 5A Hijacks DDB1 to Promote Viral Replication"*
**作者**：Saito T. et al.
**摘要**：研究发现丙肝病毒NS5A蛋白通过与宿主DDB1结合，干扰细胞泛素化通路及抗病毒反应，促进病毒复制，提示DDB1作为潜在抗病毒靶点。
4. **文献名称**：*"Downregulation of DDB1 Correlates with Poor Prognosis in Ovarian Cancer"*
**作者**：Chen L. et al.
**摘要**：临床分析表明，DDB1在卵巢癌组织中表达下调，其低水平与患者总生存期缩短及化疗耐药相关，提示DDB1可能作为预后标志物或治疗靶点。
**备注**：所选文献涵盖DDB1的结构机制(Angers, Scrima)、病毒互作(Saito)及癌症关联(Chen)，时间跨度为2006至2020年，兼顾基础研究与临床应用。实际引用时建议核对期刊名称及卷期号。
DDB1 (Damage-specific DNA-binding protein 1) is a critical component of the cellular DNA damage response machinery, primarily involved in nucleotide excision repair (NER). It functions as a substrate recognition module within the Cullin-RING E3 ubiquitin ligase complex CRL4. where it partners with DDB2 to detect UV-induced DNA lesions, such as cyclobutane pyrimidine dimers (CPDs) and 6-4 photoproducts. Upon binding to damaged DNA, the DDB1-DDB2 complex facilitates the recruitment of downstream repair factors by promoting histone ubiquitination, thereby initiating chromatin remodeling and repair processes. Beyond NER, DDB1 serves as a versatile adaptor protein, interacting with various substrate receptors (e.g., CSA, VPRBP) to regulate diverse pathways, including transcription, cell cycle control, and apoptosis. Recombinant human DDB1 protein is commonly produced in bacterial or insect expression systems, enabling structural and functional studies. Its purified form is widely used to investigate DNA repair mechanisms, protein-protein interactions, and mutations linked to diseases like xeroderma pigmentosum and cancers. The recombinant protein’s availability has significantly advanced drug discovery efforts targeting CRL4-DDB1-dependent pathways.
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