| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DCTN5 |
| Uniprot No | Q9BTE1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-182aa |
| 氨基酸序列 | MELGELLYNKSEYIETASGNKVSRQSVLCGSQNIVLNGKTIVMNDCIIRGDLANVRVGRHCVVKSRSVIRPPFKKFSKGVAFFPLHIGDHVFIEEDCVVNAAQIGSYVHVGKNCVIGRRCVLKDCCKILDNTVLPPETVVPPFTVFSGCPGLFSGELPECTQELMIDVTKSYYQKFLPLTQV |
| 分子量 | 20.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DCTN5蛋白的模拟参考文献示例(注：文献为虚构，仅作格式参考)：
1. **《Functional characterization of recombinant human DCTN5 in cytoplasmic dynein regulation》**
Smith J, et al. (2020). *Journal of Cell Biology*.
本研究通过大肠杆菌表达系统成功制备了重组人DCTN5蛋白，发现其作为动力蛋白复合体亚基在细胞内运输过程中对微管结合能力的关键调控作用。
2. **《Structural insights into DCTN5-mediated assembly of the dynactin complex》**
Chen L, et al. (2018). *Nature Structural & Molecular Biology*.
利用X射线晶体学解析了重组DCTN5蛋白的三维结构，揭示了其通过N端结构域与DCTN6相互作用，维持动力蛋白激活复合体的稳定性。
3. **《DCTN5 deficiency disrupts mitotic progression via impaired dynein localization》**
Gupta R, et al. (2021). *Molecular Biology of the Cell*.
通过CRISPR技术结合重组DCTN5回补实验，证明DCTN5缺失会导致有丝分裂期纺锤体异常，提示其在细胞分裂中的必要功能。
4. **《A novel role of DCTN5 in modulating oxidative stress response》**
Wang Y, et al. (2019). *Cell Stress & Chaperones*.
研究发现重组DCTN5蛋白通过间接调控Nrf2通路增强细胞抗氧化应激能力，为神经退行性疾病研究提供了新靶点。
如需真实文献，建议在PubMed或Google Scholar中搜索关键词：recombinant human DCTN5. DCTN5 protein function, dynactin complex.
Recombinant human DCTN5 (Dynactin subunit 5) protein is a key component of the dynactin complex, which plays a critical role in intracellular transport and mitosis by regulating dynein motor activity. DCTN5. also known as p25. stabilizes the dynactin structure by bridging other subunits, ensuring proper interaction between dynein and cargoes. Researchers produce recombinant DCTN5 using expression systems like *E. coli* or mammalian cells, followed by purification to study its biochemical properties, structural motifs, and molecular interactions. Its WD40 repeat domains mediate protein-protein interactions, essential for vesicular trafficking, organelle positioning, and spindle organization. Dysregulation of DCTN5 has been linked to neurodegenerative diseases (e.g., amyotrophic lateral sclerosis) and cancers due to impaired transport mechanisms. Recombinant DCTN5 serves as a tool for dissecting dynein-dynactin functions, screening drug candidates, and exploring pathological pathways. Studies also leverage it to generate antibodies or analyze post-translational modifications influencing cellular dynamics. This protein underscores the intersection of molecular transport and disease, driving therapeutic and diagnostic innovations.
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