| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DDEF1 |
| Uniprot No | Q9ULH1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1129aa |
| 氨基酸序列 | MRSSASRLSSFSSRDSLWNRMPDQISVSEFIAETTEDYNSPTTSSFTTRLHNCRNTVTLLEEALDQDRTALQKVKKSVKAIYNSGQDHVQNEENYAQVLDKFGSNFLSRDNPDLGTAFVKFSTLTKELSTLLKNLLQGLSHNVIFTLDSLLKGDLKGVKGDLKKPFDKAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRLFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDQKEDSQSRQGGYSMHQLQGNKEYGSEKKGYLLKKSDGIRKVWQRRKCSVKNGILTISHATSNRQPAKLNLLTCQVKPNAEDKKSFDLISHNRTYHFQAEDEQDYVAWISVLTNSKEEALTMAFRGEQSAGENSLEDLTKAIIEDVQRLPGNDICCDCGSSEPTWLSTNLGILTCIECSGIHREMGVHISRIQSLELDKLGTSELLLAKNVGNNSFNDIMEANLPSPSPKPTPSSDMTVRKEYITAKYVDHRFSRKTCSTSSAKLNELLEAIKSRDLLALIQVYAEGVELMEPLLEPGQELGETALHLAVRTADQTSLHLVDFLVQNCGNLDKQTALGNTVLHYCSMYSKPECLKLLLRSKPTVDIVNQAGETALDIAKRLKATQCEDLLSQAKSGKFNPHVHVEYEWNLRQEEIDESDDDLDDKPSPIKKERSPRPQSFCHSSSISPQDKLALPGFSTPRDKQRLSYGAFTNQIFVSTSTDSPTSPTTEAPPLPPRNAGKGPTGPPSTLPLSTQTSSGSSTLSKKRPPPPPPGHKRTLSDPPSPLPHGPPNKGAVPWGNDGGPSSSSKTTNKFEGLSQQSSTSSAKTALGPRVLPKLPQKVALRKTDHLSLDKATIPPEIFQKSSQLAELPQKPPPGDLPPKPTELAPKPQIGDLPPKPGELPPKPQLGDLPPKPQLSDLPPKPQMKDLPPKPQLGDLLAKSQTGDVSPKAQQPSEVTLKSHPLDLSPNVQSRDAIQKQASEDSNDLTPTLPETPVPLPRKINTGKNKVRRVKTIYDCQADNDDELTFIEGEVIIVTGEEDQEWWIGHIEGQPERKGVFPVSFVHILSD |
| 分子量 | 125.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是我根据相关研究领域归纳的3篇关于重组人DDEF1蛋白的模拟参考文献(请注意，实际文献需通过学术数据库查询)：
1. **《Expression and purification of recombinant human DDEF1 in E. coli for structural studies》**
- Authors: Zhang L, et al.
- 摘要：报道了通过大肠杆菌表达系统成功重组表达人源DDEF1蛋白的工艺优化，采用镍柱亲和层析和分子筛纯化获得高纯度蛋白，并通过圆二色光谱验证了其二级结构完整性。
2. **《DDEF1 interacts with ARF GTPases to regulate cell migration》**
- Authors: Miura K, et al.
- 摘要：研究发现重组DDEF1蛋白通过其PH结构域与ARF6特异性结合，调控细胞内囊泡运输和细胞迁移过程，免疫荧光实验证实其与肌动蛋白骨架的共定位。
3. **《Structural insights into the autoinhibitory mechanism of DDEF1 through cryo-EM analysis》**
- Authors: Wang X, et al.
- 摘要：利用冷冻电镜技术解析了重组DDEF1蛋白全长三维结构，发现其BAR结构域与催化域存在自抑制相互作用，此构象变化对其GAP酶活性的调控机制提供了分子解释。
建议您通过PubMed (https://pubmed.ncbi.nlm.nih.gov) 或Web of Science平台，以关键词"recombinant human DDEF1" / "ASAP1 protein" 检索最新实验研究论文。如需要特定研究方向的文献筛选，可进一步说明需求。
Recombinant human DDEF1 protein, also known as ASAP1 (ArfGAP with SH3 Domain, Ankyrin Repeat, and PH Domain 1), is a synthetically produced version of the naturally occurring DDEF1 protein encoded by the *ASAP1* gene. DDEF1 is a multidomain scaffolding protein involved in regulating diverse cellular processes, including cell migration, membrane trafficking, and signal transduction. It contains an N-terminal ArfGAP domain that hydrolyzes GTP-bound ADP-ribosylation factors (Arfs), a PH domain for membrane association, and C-terminal SH3 and ANK repeats mediating protein-protein interactions. Dysregulation of DDEF1 expression is linked to cancer progression, as it promotes tumor cell invasion, metastasis, and angiogenesis by modulating cytoskeletal dynamics and integrin signaling pathways. Recombinant DDEF1 is typically expressed in *E. coli* or mammalian systems, purified via affinity tags (e.g., His-tag), and used to study its biochemical properties, structure-function relationships, and role in cellular mechanisms. It serves as a critical tool in drug discovery targeting DDEF1-associated pathways in oncology.
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