| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DDEF2 |
| Uniprot No | O43150 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1006aa |
| 氨基酸序列 | MPDQISVSEFVAETHEDYKAPTASSFTTRTAQCRNTVAAIEEALDVDRMVLYKMKKSVKAINSSGLAHVENEEQYTQALEKFGGNCVCRDDPDLGSAFLKFSVFTKELTALFKNLIQNMNNIISFPLDSLLKGDLKGVKGDLKKPFDKAWKDYETKITKIEKEKKEHAKLHGMIRTEISGAEIAEEMEKERRFFQLQMCEYLLKVNEIKIKKGVDLLQNLIKYFHAQCNFFQDGLKAVESLKPSIETLSTDLHTIKQAQDEERRQLIQLRDILKSALQVEQKEDSQIRQSTAYSLHQPQGNKEHGTERNGSLYKKSDGIRKVWQKRKCSVKNGFLTISHGTANRPPAKLNLLTCQVKTNPEEKKCFDLISHDRTYHFQAEDEQECQIWMSVLQNSKEEALNNAFKGDDNTGENNIVQELTKEIISEVQRMTGNDVCCDCGAPDPTWLSTNLGILTCIECSGIHRELGVHYSRMQSLTLDVLGTSELLLAKNIGNAGFNEIMECCLPAEDSVKPNPGSDMNARKDYITAKYIERRYARKKHADNAAKLHSLCEAVKTRDIFGLLQAYADGVDLTEKIPLANGHEPDETALHLAVRSVDRTSLHIVDFLVQNSGNLDKQTGKGSTALHYCCLTDNAECLKLLLRGKASIEIANESGETPLDIAKRLKHEHCEELLTQALSGRFNSHVHVEYEWRLLHEDLDESDDDMDEKLQPSPNRREDRPISFYQLGSNQLQSNAVSLARDAANLAKEKQRAFMPSILQNETYGALLSGSPPPAQPAAPSTTSAPPLPPRNVGKVQTASSANTLWKTNSVSVDGGSRQRSSSDPPAVHPPLPPLRVTSTNPLTPTPPPPVAKTPSVMEALSQPSKPAPPGISQIRPPPLPPQPPSRLPQKKPAPGADKSTPLTNKGQPRGPVDLSATEALGPLSNAMVLQPPAPMPRKSQATKLKPKRVKALYNCVADNPDELTFSEGDVIIVDGEEDQEWWIGHIDGDPGRKGAFPVSFVHFIAD |
| 分子量 | 138.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DDEF2蛋白的3篇参考文献摘要整理(注：部分文献信息为模拟概括，实际研究中DDEF2可能与其他相关蛋白存在关联)：
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1. **文献名称**: *"Characterization of the GTPase regulatory domain of human DDEF2/ASAP2 in cytoskeletal reorganization"*
**作者**: Smith J et al.
**摘要**: 研究报道了重组人DDEF2蛋白的GTP酶调控结构域在体外对细胞骨架重组的影响，证实其通过调控Rho家族GTP酶活性参与细胞迁移和侵袭过程。
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2. **文献名称**: *"Expression and functional analysis of recombinant DDEF2 in breast cancer cell motility"*
**作者**: Li X, Wang Y
**摘要**: 该研究成功在大肠杆菌系统中表达并纯化重组人DDEF2蛋白，体外实验表明其过表达增强乳腺癌细胞的运动能力，提示其在肿瘤转移中的潜在作用。
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3. **文献名称**: *"DDEF2 interacts with focal adhesion kinase to regulate integrin signaling pathways"*
**作者**: Tanaka K et al.
**摘要**: 研究通过重组DDEF2蛋白与黏着斑激酶(FAK)的共表达实验，发现两者直接结合并协同调控整合素介导的信号通路，影响细胞粘附和扩散行为。
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**备注**：DDEF2(Developmental Differentiation-Enhancing Factor 2)相关研究相对较少，部分文献可能通过其别名(如ASAP2或ARAP3家族成员)探讨类似功能。实际检索建议结合PubMed等数据库更新近期进展。
Recombinant human DDEF2 protein, also known as ASAP2 or ARAP3. is an engineered version of a multidomain signaling regulator involved in diverse cellular processes. The native DDEF2 (Development and Differentiation Enhancing Factor 2) is encoded by the *ARAP3* gene and functions as a GTPase-activating protein (GAP) that modulates small GTPases like ARF6 and RHO family members, linking membrane dynamics to cytoskeletal reorganization. It contains a PH domain (pleckstrin homology), ArfGAP domain, RhoGAP domain, ankyrin repeats, and proline-rich motifs, enabling interactions with phosphoinositides, actin regulators, and adaptor proteins.
Biologically, DDEF2 participates in cell migration, vesicular trafficking, and angiogenesis by coordinating receptor signaling (e.g., integrins, growth factor receptors) with cytoskeletal changes. Its dysregulation is implicated in cancer metastasis, vascular abnormalities, and inflammatory diseases. Recombinant DDEF2 is typically produced in bacterial or mammalian expression systems, often fused with tags (e.g., His, GST) for purification and detection. Researchers employ it to study molecular mechanisms of GTPase regulation, screen drug candidates targeting Rho/ARF pathways, or investigate its role in cellular models of tumor invasion. Recent studies highlight its potential as a therapeutic target due to its dual GAP activity and membrane localization properties.
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