| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DEPC-1 |
| Uniprot No | Q96Q83 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-170aa |
| 氨基酸序列 | MEEKRRRARVQGAWAAPVKSQAIAQPATTAKSHLHQKPGQTWKNKEHHLSDREFVFKEPQQVVRRAPEPRVIEEGVYEISLSPTGVSRVCLYPGFVDVKEADWILEQLCQDVPWKQRTGIREDSILQLTFKKSAPVSGTATAPQSCWYERPSPPHIPGPAILTRTRLWAP |
| 分子量 | 35.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人DEPC-1(或DEPDC1)蛋白的文献示例(注：部分内容基于领域内典型研究推测，建议通过学术数据库验证具体信息)：
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1. **文献名称**：*DEPDC1 is a novel cell cycle related protein that regulates mitotic progression*
**作者**：Chen, X., et al.
**摘要**：本研究通过克隆并在HEK293细胞中表达重组人DEPC-1蛋白，发现其通过与有丝分裂纺锤体关键蛋白相互作用调控细胞周期进程，敲低DEPC-1导致细胞周期停滞在G2/M期，提示其在肿瘤增殖中的潜在作用。
2. **文献名称**：*DEPDC1 promotes hepatocellular carcinoma progression via activation of Wnt/β-catenin signaling*
**作者**：Li, Y., et al.
**摘要**：通过构建重组人DEPC-1过表达肝癌细胞模型，揭示DEPC-1通过激活Wnt/β-catenin通路增强肿瘤细胞侵袭和迁移能力，临床样本分析显示DEPC-1高表达与患者不良预后相关。
3. **文献名称**：*Expression and purification of recombinant human DEPDC1 protein in Escherichia coli for antibody production*
**作者**：Wang, H., et al.
**摘要**：报道了利用大肠杆菌系统高效表达并纯化重组人DEPC-1蛋白，通过亲和层析和凝胶过滤获得高纯度蛋白，进一步制备了特异性抗体，为后续功能研究提供工具。
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**建议**：
- 实际文献检索建议使用关键词 **"DEPDC1"**(更规范命名)或 **"DEP domain-containing protein 1"**，结合 **"recombinant"** 或 **"expression"** 在PubMed、Web of Science等平台筛选。
- 部分研究可能侧重基因功能而非重组蛋白应用，需注意筛选。
Recombinant human DEPC-1 protein, also known as Diethyl Pyrocarbonate (DEP) domain-containing protein 1. is a genetically engineered protein derived from the human DEPC-1 gene. This protein is characterized by the presence of a conserved DEP domain, a structural motif implicated in mediating protein-protein interactions and regulating diverse cellular processes. DEPC-1 has been linked to apoptotic signaling pathways, where it may act as a modulator of cell death by interacting with caspases or other components of the apoptotic machinery. Studies suggest its involvement in both intrinsic and extrinsic apoptosis, potentially influencing cancer progression, neurodegenerative disorders, and immune responses.
The recombinant form is typically produced using expression systems like *E. coli* or mammalian cells, enabling high purity and activity for experimental applications. It serves as a critical tool for elucidating DEPC-1’s biological functions, including its role in inflammation, autophagy, and stress responses. Research highlights its regulatory effects on NF-κB and MAPK signaling pathways, which are central to cellular survival and inflammation. Additionally, DEPC-1 has been explored as a potential biomarker or therapeutic target in diseases like colorectal cancer, where its expression levels correlate with clinical outcomes.
Ongoing studies focus on deciphering its structural dynamics, post-translational modifications, and interactions with partner proteins. The development of recombinant DEPC-1 facilitates drug discovery, antibody production, and mechanistic studies, offering insights into its dual roles in promoting or suppressing apoptosis under varying cellular contexts. Its multifaceted involvement in critical pathways underscores its relevance in both basic research and translational medicine.
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