| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DNAJA2 |
| Uniprot No | O60884 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-412aa |
| 氨基酸序列 | MANVADTKLYDILGVPPGASENELKKAYRKLAKEYHPDKNPNAGDKFKEISFAYEVLSNPEKRELYDRYGEQGLREGSGGGGGMDDIFSHIFGGGLFGFMGNQSRSRNGRRRGEDMMHPLKVSLEDLYNGKTTKLQLSKNVLCSACSGQGGKSGAVQKCSACRGRGVRIMIRQLAPGMVQQMQSVCSDCNGEGEVINEKDRCKKCEGKKVIKEVKILEVHVDKGMKHGQRITFTGEADQAPGVEPGDIVLLLQEKEHEVFQRDGNDLHMTYKIGLVEALCGFQFTFKHLDGRQIVVKYPPGKVIEPGCVRVVRGEGMPQYRNPFEKGDLYIKFDVQFPENNWINPDRLSELEDLLPSRPEVPNIIGETEEVELQEFDSTRGSGGGQRREAYNDSSDEESSSHHGPGVQCAHQ |
| 分子量 | 71.06 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DNAJA2蛋白的3篇参考文献的简要概述(注：文献为示例性描述，可能不对应真实发表的研究)：
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1. **文献名称**: *"Expression and functional characterization of recombinant human DNAJA2 in a bacterial system"*
**作者**: Zhang Y. et al.
**摘要**: 本研究成功在大肠杆菌中表达并纯化了重组人DNAJA2蛋白，验证其通过结合Hsp70协同促进底物蛋白的正确折叠，并揭示了其C端结构域在调控分子伴侣活性中的关键作用。
2. **文献名称**: *"DNAJA2 modulates mitochondrial proteostasis and cellular oxidative stress response"*
**作者**: Kumar R. et al.
**摘要**: 研究通过重组DNAJA2蛋白的功能实验，发现其通过维持线粒体蛋白稳态减少氧化应激损伤，敲低DNAJA2导致线粒体复合物功能障碍和活性氧(ROS)积累，提示其在神经退行性疾病中的潜在作用。
3. **文献名称**: *"DNAJA2 interacts with mutant p53 to promote cancer cell survival"*
**作者**: Lee S. et al.
**摘要**: 利用重组DNAJA2进行互作分析，发现其与突变型p53蛋白结合并抑制泛素化降解，增强癌症细胞的化疗耐药性，为靶向DNAJA2-p53通路的抗肿瘤策略提供了依据。
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以上内容综合了DNAJA2在重组表达、分子机制及疾病关联中的典型研究方向，涵盖蛋白质折叠、应激反应和肿瘤生物学等角度。实际文献需通过学术数据库检索具体信息。
**Background of Recombinant Human DNAJA2 Protein**
DNAJA2. a member of the Hsp40/DnaJ protein family, functions as a co-chaperone to regulate the activity of Hsp70 chaperones. It plays a critical role in protein quality control by aiding in the folding, stabilization, and degradation of client proteins. Structurally, DNAJA2 contains a conserved J-domain that interacts with Hsp70. along with substrate-binding domains to facilitate ATPase activity and substrate transfer.
Expressed ubiquitously in tissues, DNAJA2 localizes to the cytoplasm and endoplasmic reticulum, where it participates in stress responses and maintains proteostasis. Dysregulation of DNAJA2 has been linked to diseases, including cancer and neurodegenerative disorders, due to its influence on cell proliferation, apoptosis, and metastasis-related pathways.
Recombinant human DNAJA2 protein is engineered using expression systems (e.g., *E. coli* or mammalian cells*) to produce high-purity, functional protein for research. It retains chaperone activity, enabling studies on protein misfolding diseases, cancer mechanisms, and therapeutic development. Tags (e.g., His-tag) are often added for purification and detection. Research on recombinant DNAJA2 enhances understanding of cellular stress responses and provides potential targets for disease intervention.
Current studies focus on its interactions with oncoproteins, role in viral replication, and modulation of signaling pathways, highlighting its biomedical relevance.
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