| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DYNC1I2 |
| Uniprot No | Q13409 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-612aa |
| 氨基酸序列 | MSDKSELKAELERKKQRLAQIREEKKRKEEERKKKETDQKKEAVAPVQEESDLEKKRREAEALLQSMGLTPESPIVPPPMSPSSKSVSTPSEAGSQDSGDGAVGSRRGPIKLGMAKITQVDFPPREIVTYTKETQTPVMAQPKEDEEEDDDVVAPKPPIEPEEEKTLKKDEENDSKAPPHELTEEEKQQILHSEEFLSFFDHSTRIVERALSEQINIFFDYSGRDLEDKEGEIQAGAKLSLNRQFFDERWSKHRVVSCLDWSSQYPELLVASYNNNEDAPHEPDGVALVWNMKYKKTTPEYVFHCQSAVMSATFAKFHPNLVVGGTYSGQIVLWDNRSNKRTPVQRTPLSAAAHTHPVYCVNVVGTQNAHNLISISTDGKICSWSLDMLSHPQDSMELVHKQSKAVAVTSMSFPVGDVNNFVVGSEEGSVYTACRHGSKAGISEMFEGHQGPITGIHCHAAVGAVDFSHLFVTSSFDWTVKLWTTKNNKPLYSFEDNADYVYDVMWSPTHPALFACVDGMGRLDLWNLNNDTEVPTASISVEGNPALNRVRWTHSGREIAVGDSEGQIVIYDVGEQIAVPRNDEWARFGRTLAEINANRADAEEEAATRIPA |
| 分子量 | 94.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DYNC1I2蛋白的3篇参考文献示例(注：文献标题和作者为虚构示例，仅供格式参考)：
1. **文献名称**："Functional characterization of recombinant human DYNC1I2 in cytoplasmic dynein complex assembly"
**作者**：Smith A, et al.
**摘要**：研究重组DYNC1I2蛋白在体外与动力蛋白重链和轻链的相互作用，证实其作为中间链在复合体组装中的支架作用，并通过突变分析鉴定关键结构域。
2. **文献名称**："Expression and purification of human DYNC1I2 for structural studies using baculovirus system"
**作者**：Chen L, et al.
**摘要**：利用杆状病毒表达系统高效表达重组DYNC1I2蛋白，优化纯化流程获得高纯度蛋白，并通过冷冻电镜解析其与动力蛋白复合体的结构。
3. **文献名称**："DYNC1I2 knockdown disrupts Golgi apparatus trafficking: Insights from recombinant protein rescue experiments"
**作者**：Wang X, et al.
**摘要**：通过siRNA敲低DYNC1I2导致高尔基体运输异常，外源性添加重组DYNC1I2蛋白可部分恢复运输功能，表明其在细胞器定位中的必要性。
**注意事项**：实际文献需在PubMed、Google Scholar等平台以关键词“recombinant DYNC1I2”、“human cytoplasmic dynein intermediate chain”检索，并筛选涉及蛋白表达、功能或应用的论文。
Recombinant human DYNC1I2 protein is a engineered version of the cytoplasmic dynein 1 intermediate chain 2. a critical component of the dynein motor complex involved in intracellular transport and mitosis. DYNC1I2. encoded by the *DYNC1I2* gene, functions as part of the multi-subunit dynein complex that drives minus-end-directed movement along microtubules, facilitating organelle positioning, vesicle trafficking, and chromosomal segregation. This intermediate chain plays a structural role in linking the dynein heavy chain to light chains and regulatory proteins, ensuring cargo specificity and complex stability.
The recombinant form is typically produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) for biochemical and functional studies. Its production enables researchers to investigate dynein-mediated transport mechanisms, microtubule interactions, and roles in neurodevelopment, as DYNC1I2 mutations have been implicated in neurodevelopmental disorders. Recombinant DYNC1I2 is also utilized in drug discovery to screen compounds targeting dynein dysfunction in neurodegenerative diseases or cancer, where altered dynein activity affects cell division and metastasis. Studies using this recombinant protein have advanced understanding of intracellular logistics and disease pathways, offering potential therapeutic insights.
×
