| 纯度 | > 90 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | CBX5 |
| Uniprot No | P45973 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-191aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSHMGKKTK RTADSSSSED EEEYVVEKVL DRRVVKGQVE YLLKWKGFSE EHNTWEPEKN LDCPELISEF MKKYKKMKEG ENNKPREKSE SNKRKSNFSN SADDIKSKKK REQSNDIARG FERGLEPEKI IGATDSCGDL MFLMKWKDTD EADLVLAKEA NVKCPQIVIA FYEERLTWHA YPEDAENKEK ETAKS |
| 预测分子量 | 25 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CBX5重组蛋白的3篇代表性文献示例(内容基于真实研究领域方向概括，作者及标题为虚构示例)：
1. **文献名称**：*"重组CBX5蛋白的异源表达及其与组蛋白H3K9me3的相互作用研究"*
**作者**：Zhang et al.
**摘要**：本研究通过大肠杆菌表达系统成功纯化重组CBX5蛋白，并利用表面等离子共振(SPR)技术证实其与组蛋白H3K9三甲基化(H3K9me3)的特异性结合，揭示了CBX5在异染色质形成中的分子机制。
2. **文献名称**：*"CBX5重组蛋白的结构解析及其在基因沉默中的功能验证"*
**作者**：Li et al.
**摘要**：通过X射线晶体学解析了重组CBX5蛋白的晶体结构，发现其chromodomain结构域的关键氨基酸残基对结合H3K9me3至关重要。细胞实验进一步证明重组CBX5可恢复HP1α缺失细胞的异染色质稳定性。
3. **文献名称**：*"基于重组CBX5的高通量筛选平台开发及其抗癌药物发现应用"*
**作者**：Wang et al.
**摘要**：构建了以重组CBX5蛋白为核心的体外筛选模型，用于靶向HP1蛋白-染色质相互作用的抑制剂筛选，成功鉴定了两种小分子化合物可破坏CBX5与染色质结合，为癌症表观遗传治疗提供新策略。
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**注**：以上文献为示例性概括，实际研究需参考具体论文(如Nature、Cell中HP1蛋白相关文章)。真实文献可通过PubMed搜索“CBX5 recombinant”或“HP1α expression”获取。
CBX5 (Chromobox Homolog 5), also known as HP1α (Heterochromatin Protein 1α), is a key epigenetic regulator belonging to the Polycomb group (PcG) protein family. It plays a central role in maintaining heterochromatin structure and gene silencing through its interaction with trimethylated lysine 27 on histone H3 (H3K27me3), a hallmark of repressive chromatin marks. Structurally, CBX5 contains a conserved chromodomain that recognizes H3K9me3 (trimethylated histone H3 lysine 9) and a chromoshadow domain mediating protein-protein interactions. This dual functionality enables CBX5 to bridge chromatin modifications with effector proteins, facilitating transcriptional repression and genomic stability.
Recombinant CBX5 protein is engineered using expression systems like *E. coli* or mammalian cells to produce purified, functional protein for research. It retains the ability to bind modified histones and recruit downstream partners, making it invaluable for studying heterochromatin formation, gene regulation, and epigenetic inheritance. Researchers utilize CBX5 recombinant protein in assays such as chromatin immunoprecipitation (ChIP), *in vitro* binding studies, and mechanistic investigations of Polycomb repressive complex 1 (PRC1) function.
CBX5 dysregulation has been implicated in cancers, developmental disorders, and aging-related diseases. Its overexpression is linked to tumor progression, while loss of function disrupts differentiation and cellular identity. Recombinant CBX5 serves as a tool for drug discovery targeting epigenetic pathways and for deciphering its role in stem cell pluripotency, X-chromosome inactivation, and viral latency. Its study advances therapeutic strategies for diseases driven by epigenetic instability.
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