| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EFCAB4A |
| Uniprot No | Q8N4Y2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-294aa |
| 氨基酸序列 | MASPGKPGADEAQEEEGELEGGSAGPRAAILEQAEELFLLCDKEAKGFITKHDLQGLQSDLPLTPEQLEAVFESLDQAHTGFLTAREFCLGLGMFVGVASAQGANPCRTPEETFESGGLDVQGTAGSLDEEEEEEERFHTVLEQLGVAPVLGEQRAVRTLWARLQRERPELLGSFEDVLIRASACLEEAARERDGLEQALRRRESEHEREVRALYEETEQLREQSRRPPSQNFARGERRSRLELELQIREQDLERAGLRQRERRGRRLQEHAERESQPATATGAAQGAEYTAAG |
| 分子量 | 59.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人EFCAB4A蛋白的参考文献示例(注：部分信息为示例性虚构，实际文献需通过数据库验证)：
1. **"Expression and purification of recombinant human EFCAB4A in Escherichia coli"**
*作者：Zhang Y. et al.*
**摘要**：本研究成功构建了人EFCAB4A的重组表达载体，并利用大肠杆菌系统高效表达可溶性蛋白，优化了纯化条件，为后续功能研究提供基础。
2. **"Structural insights into calcium-binding properties of EFCAB4A through cryo-EM analysis"**
*作者：Lee S. & Tanaka H.*
**摘要**：通过冷冻电镜解析了重组人EFCAB4A蛋白的钙结合结构域，揭示其EF-hand基序特异性结合钙离子的分子机制。
3. **"EFCAB4A regulates ciliary motility via calcium signaling in human respiratory epithelia"**
*作者：Chen L. et al.*
**摘要**：研究发现重组人EFCAB4A蛋白通过调控纤毛细胞钙离子动态影响纤毛运动，可能为治疗纤毛功能障碍疾病提供靶点。
4. **"EFCAB4A overexpression promotes tumor metastasis by activating Wnt/β-catenin pathway"**
*作者：Patel R. & Wang Q.*
**摘要**：利用重组EFCAB4A蛋白证实其在乳腺癌中异常表达，通过激活Wnt通路促进肿瘤转移，提示其作为潜在治疗标记物。
建议通过PubMed或Web of Science搜索“recombinant human EFCAB4A”获取真实文献。
Recombinant human EFCAB4A protein is a genetically engineered variant of the EFCAB4A (EF-hand calcium-binding domain-containing protein 4A) encoded by the human EFCAB4A gene. This protein belongs to the EF-hand calcium-binding protein family, characterized by conserved helix-loop-helix motifs that bind calcium ions, enabling roles in calcium-dependent signaling pathways. EFCAB4A is relatively less studied compared to other EF-hand proteins, but emerging research suggests its involvement in cellular processes such as protein trafficking, cytoskeletal organization, and modulation of ion channel activity. It has been implicated in regulating intracellular calcium homeostasis, potentially influencing cell motility, apoptosis, or secretion mechanisms.
The recombinant form is typically produced using expression systems like *E. coli* or mammalian cells, followed by purification via affinity chromatography to ensure high purity and bioactivity. Its recombinant production enables functional studies, including structural analysis, interaction partner identification, and mechanistic exploration of its role in diseases. For instance, dysregulation of calcium signaling linked to EFCAB4A has been associated with cancer progression and neurological disorders, making it a potential biomarker or therapeutic target. Current research focuses on elucidating its precise molecular interactions and contribution to pathological conditions, leveraging recombinant EFCAB4A as a tool for *in vitro* and *in vivo* experiments. Further studies are needed to fully unravel its physiological and pathological significance.
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