| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EFCAB4B |
| Uniprot No | Q9BSW2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-395aa |
| 氨基酸序列 | MAAPDGRVVSRPQRLGQGSGQGPKGSGACLHPLDSLEQKETQEQTSGQLVMLRKAQEFFQTCDAEGKGFIARKDMQRLHKELPLSLEELEDVFDALDADGNGYLTPQEFTTGFSHFFFSQNNPSQEDAGEQVAQRHEEKVYLSRGDEDLGDMGEDEEAQFRMLMDRLGAQKVLEDESDVKQLWLQLKKEEPHLLSNFEDFLTRIISQLQEAHEEKNELECALKRKIAAYDEEIQHLYEEMEQQIKSEKEQFLLKDTERFQARSQELEQKLLCKEQELEQLTQKQKRLEGQCTALHHDKHETKAENTKLKLTNQELARELERTSWELQDAQQQLESLQQEACKLHQEKEMEVYRVTESLQREKAGLLKQLDFLRCVGGHWPVLRAPPRSLGSEGPV |
| 分子量 | 72 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人EFCAB4B蛋白的3篇参考文献示例(注：文献为假设性模拟，实际需根据真实文献调整)：
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1. **文献名称**: "Recombinant expression and structural analysis of human EFCAB4B calcium-binding protein"
**作者**: Zhang L, et al. (2021)
**摘要**: 本研究首次通过大肠杆菌系统重组表达了人源EFCAB4B蛋白，利用圆二色谱和X射线晶体学揭示了其EF-hand结构域中钙离子结合的特定位点，证实其参与细胞钙信号调控的分子基础。
2. **文献名称**: "EFCAB4B interacts with HIF-1α to modulate hypoxia responses in breast cancer cells"
**作者**: Wang Y, et al. (2020)
**摘要**: 通过免疫共沉淀和基因沉默实验发现，重组EFCAB4B蛋白在乳腺癌细胞中与HIF-1α直接结合，调控缺氧诱导的肿瘤转移通路，提示其可能作为癌症治疗的潜在靶点。
3. **文献名称**: "Functional screening of EFCAB4B mutations in neurological disorders"
**作者**: Smith JR, et al. (2019)
**摘要**: 本研究利用重组EFCAB4B蛋白构建突变体库，筛选发现两个错义突变(R128C和G215D)导致蛋白稳定性下降，并与自闭症谱系障碍患者的基因变异关联，暗示其在神经发育中的功能。
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如需真实文献，建议通过PubMed或Google Scholar检索关键词 **"EFCAB4B"** 或 **"EF-hand calcium-binding protein 4B"** 获取最新研究。
Recombinant human EFCAB4B protein is a calcium-binding protein belonging to the EF-hand superfamily, which plays roles in calcium-mediated signaling pathways. EFCAB4B (EF-hand calcium-binding domain-containing protein 4B) is encoded by the EFCAB4B gene and is involved in modulating intracellular calcium homeostasis, a critical regulator of diverse cellular processes including enzyme activity, gene expression, and membrane trafficking. Structurally, it contains conserved EF-hand motifs that enable calcium ion coordination, facilitating interactions with target proteins or membranes. While its exact biological functions remain under investigation, emerging studies link EFCAB4B to cancer progression, neural development, and immune responses. For instance, it may influence tumor cell proliferation or apoptosis by regulating calcium-dependent signaling cascades. Recombinant EFCAB4B is typically produced using expression systems like Escherichia coli or mammalian cells, ensuring proper post-translational modifications. Purified recombinant proteins serve as tools for in vitro studies, such as deciphering molecular interactions, calcium-binding assays, or screening therapeutic candidates. Research on EFCAB4B may deepen understanding of calcium signaling networks and their implications in disease mechanisms, potentially aiding drug development targeting calcium-related disorders.
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