| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EFHC2 |
| Uniprot No | Q5JST6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-749aa |
| 氨基酸序列 | MALPLLPGNS FNRNVGKEKF HKSQHWGFCN NVMMLVSDEK PGIGGEPLLG QKIKPKCSIY PKGDGSDVPS WVAFDKQVLS FDAYLEEEVL DKSQTNYRIR YYKIYFYPED DTIQVNEPEV KNSGLLQGTS IRRHRITLPP PDEDQFYTVY HFNVGTEVVF YGRTFKIYDC DAFTRNFLRK IGVKVNPPVQ CPEDPYMKIR REVVEHVEPL RPYESLDTLK QFLQYHGKIL CFFCLWDDSV SMFGDRRELI LHYFLCDDTI EIKELLPHSS GRDALKMFLR RSKLPKNCPP RVYQPGQITD RAVLNSYGDF IKNQADGYLF DRYKLGKVDQ EFYKDSDLSL GVTINVWGRK VLLYDCDEFT KSYYKSKYGI ENFTSVSCKP PSPPPKIERK FPPYNGFGSE EDSLRNCIDL KPTPHRRNFK KFMEKDSYGS KSNILRFFAK LVTDKCVDLD RMFVISYYLG DDTISVFEPI ERNSGIAGGM FLKRSRVKKP GQEVFKSELS EYIKAEELYI GVTVNVNGYL FRLLNADEYT LNYMEQNTDK YPFSNLKLAL QKLKQEEGKS RELKQVFKAA DSKHTNMVDY NTFRDILMSL TVGNLAEQEF VTIARHYRVP EGTCSDMDFL IALAHEKFKK NMFENFDTFI YSCVYEDREK KNVLPTKDIK RLCKSSRLPL SDDLLESLLS RFEDSEKQID YKSFFSALNW RKNPVPELQP ASYLKERCED VWLGMPSPIP AKYIDYWTFL KDAFGLEEE |
| 分子量 | 87.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是模拟生成的关于重组人EFHC2蛋白的研究参考文献示例，供参考：
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1. **文献名称**: *Cloning, Expression, and Functional Analysis of Human EFHC2 Protein*
**作者**: Yamamoto A. et al.
**摘要**: 本研究成功克隆并重组表达了人EFHC2蛋白，使用大肠杆菌表达系统获得可溶性蛋白。通过体外实验发现EFHC2具有钙离子依赖的蛋白质结合活性，并可能在细胞周期调控中发挥作用。
2. **文献名称**: *EFHC2 Suppresses Tumor Cell Migration by Interacting with Microtubule Networks*
**作者**: Li X. et al.
**摘要**: 通过重组EFHC2蛋白的体外功能实验，发现其能够与微管蛋白相互作用，抑制癌细胞的迁移能力。进一步机制研究表明，EFHC2可能通过调节细胞骨架动力学发挥肿瘤抑制作用。
3. **文献名称**: *Structural Insights into the Calcium-Binding Motifs of EFHC2*
**作者**: Sato K. et al.
**摘要**: 利用X射线晶体学解析了重组EFHC2蛋白的EF-hand结构域三维结构，揭示了其钙离子结合位点的独特构象，为研究其在信号传导中的作用提供了结构基础。
4. **文献名称**: *EFHC2 Knockdown Induces Apoptosis in Neuronal Cells*
**作者**: Chen L. et al.
**摘要**: 研究发现重组EFHC2蛋白在神经元细胞中通过调节线粒体膜电位影响凋亡通路。RNA干扰实验表明EFHC2缺失导致细胞凋亡率显著上升，提示其在神经退行性疾病中的潜在功能。
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**注意**：以上文献信息为模拟生成，实际研究请通过PubMed、Web of Science或Google Scholar等学术平台查询关键词“EFHC2”“recombinant EFHC2”“EF-hand domain”等获取。
Recombinant human EFHC2 protein is a genetically engineered form of the EF-hand domain-containing protein 2 (EFHC2), which belongs to the EF-hand calcium-binding protein family. EFHC2 is characterized by its EF-hand motifs, structural domains that enable calcium ion binding, suggesting its potential role in calcium-mediated signaling pathways. This protein is encoded by the EFHC2 gene, located on chromosome X, and is expressed in various tissues, including the brain, testis, and skeletal muscle. While its exact physiological functions remain under investigation, EFHC2 is hypothesized to participate in cellular processes such as apoptosis, cell cycle regulation, and neuronal development. Dysregulation of EFHC2 has been implicated in neurological disorders, including epilepsy and neurodevelopmental conditions, as well as certain cancers, though mechanistic insights are still emerging.
Recombinant EFHC2 is typically produced using expression systems like *E. coli* or mammalian cell lines to ensure proper folding and post-translational modifications. It serves as a critical tool for studying EFHC2's interactions, structure-function relationships, and downstream signaling mechanisms in vitro or in vivo. Researchers employ it in assays to identify binding partners, map enzymatic activities, or screen therapeutic compounds targeting EFHC2-associated pathways. Challenges in its application include optimizing solubility and maintaining functional integrity during purification. Current studies focus on elucidating its role in calcium signaling cross-talk, disease pathogenesis, and its potential as a biomarker or drug target. Further exploration of EFHC2 could advance understanding of its dual roles in cellular homeostasis and disease contexts.
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