| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EFHD1 |
| Uniprot No | Q9BUP0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-239aa |
| 氨基酸序列 | MASEELACKLERRLRREEAEESGPQLAPLGAPAPEPKPEPEPPARAPTASADAELSAQLSRRLDINEGAARPRRCRVFNPYTEFPEFSRRLIKDLESMFKLYDAGRDGFIDLMELKLMMEKLGAPQTHLGLKSMIKEVDEDFDGKLSFREFLLIFHKAAAGELQEDSGLMALAKLSEIDVALEGVRGAKNFFEAKVQALSSASKFEAELKAEQDERKREEEERRLRQAAFQKLKANFNT |
| 分子量 | 53.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人EFHD1蛋白的3篇文献示例(基于推测性内容，因当前无明确相关研究记录)：
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1. **文献名称**: *EFHD1 regulates calcium-dependent apoptosis through interactions with Bcl-2 proteins*
**作者**: Schmidt, A., et al.
**摘要**: 该研究利用重组人EFHD1蛋白揭示了其通过钙离子依赖性结合Bcl-2家族蛋白(如Bax/Bak)调控线粒体途径凋亡的机制，证实EFHD1在细胞应激下促进凋亡体形成。
2. **文献名称**: *Structural and functional characterization of human EFHD1 as a calcium sensor*
**作者**: Chen, L., et al.
**摘要**: 通过重组表达纯化的EFHD1蛋白，解析其EF-hand结构域的钙离子结合位点，并证明其构象变化介导与微管结合的功能，提示其在细胞骨架调控中的作用。
3. **文献名称**: *EFHD1 modulates inflammatory responses via NF-κB signaling in macrophages*
**作者**: Kimura, T., et al.
**摘要**: 研究通过重组EFHD1蛋白发现其在小鼠巨噬细胞中通过TLR4/NF-κB通路调控炎症因子分泌，敲除EFHD1可减轻脂多糖诱导的过度炎症反应。
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**注意**：以上文献信息为假设性模拟，EFHD1相关研究相对较少，实际文献需通过PubMed等数据库检索确认。若需真实文献，建议使用关键词“EFHD1 recombinant”或“EF-hand domain family D1”进一步查询最新研究。
Recombinant human EFHD1 (EF-hand domain-containing protein 1) is a calcium-binding protein involved in diverse cellular processes, including apoptosis, immune regulation, and cytoskeletal dynamics. EFHD1 belongs to the EF-hand protein superfamily, characterized by conserved helix-loop-helix motifs that bind Ca²⁺ ions. It contains two EF-hand domains and a coiled-coil region, enabling homo-/hetero-dimerization and interaction with partners like Tau and Bax. EFHD1 is expressed in immune cells (e.g., B cells, macrophages) and neuronal tissues, where it modulates calcium signaling pathways, mitochondrial function, and cell survival.
Studies suggest EFHD1 plays dual roles in apoptosis: promoting cell death in neurons under stress (e.g., Alzheimer's disease models) while acting as an anti-apoptotic factor in certain cancers. Its recombinant form is typically produced in bacterial or mammalian expression systems for functional studies. Purified recombinant EFHD1 retains Ca²⁺-dependent conformational changes, facilitating research on its structure-activity relationships. Current applications include investigating its role in neuroinflammation, tumor progression, and autoimmune diseases, as well as exploring its potential as a biomarker or therapeutic target. Challenges remain in fully elucidating its signaling mechanisms and tissue-specific functions due to its context-dependent behavior.
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