| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EPS8L3 |
| Uniprot No | Q8TE67 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-594aa |
| 氨基酸序列 | MSRPSSRAIYLHRKEYSQNLTSEPTLLQHRVEHLMTCKQGSQRVQGPEDALQKLFEMDAQGRVWSQDLILQVRDGWLQLLDIETKEELDSYRLDSIQAMNVALNTCSYNSILSITVQEPGLPGTSTLLFQCQEVGAERLKTSLQKALEEELEQSRPRLGGLQPGQDRWRGPAMERPLPMEQARYLEPGIPPEQPHQRTLEHSLPPSPRPLPRHTSAREPSAFTLPPPRRSSSPEDPERDEEVLNHVLRDIELFMGKLEKAQAKTSRKKKFGKKNKDQGGLTQAQYIDCFQKIKYSFNLLGRLATWLKETSAPELVHILFKSLNFILARCPEAGLAAQVISPLLTPKAINLLQSCLSPPESNLWMGLGPAWTTSRADWTGDEPLPYQPTFSDDWQLPEPSSQAPLGYQDPVSLRRGSHRLGSTSHFPQEKTHNHDPQPGDPNSRPSSPKPAQPALKMQVLYEFEARNPRELTVVQGEKLEVLDHSKRWWLVKNEAGRSGYIPSNILEPLQPGTPGTQGQSPSRVPMLRLSSRPEEVTDWLQAENFSTATVRTLGSLTGSQLLRIRPGELQMLCPQEAPRILSRLEAVRRMLGISP |
| 分子量 | 93.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人EPS8L3蛋白的3篇示例参考文献(注：文献信息为虚构模拟，仅供示例参考)：
1. **"Structural and functional characterization of human EPS8L3 in actin remodeling"**
*Authors: Chen L, et al.*
摘要：本研究通过X射线晶体学解析了重组人EPS8L3蛋白的分子结构，发现其C端结构域通过与肌动蛋白结合调控细胞骨架重塑，揭示其在细胞运动中的关键作用。
2. **"EPS8L3 as a potential biomarker in colorectal cancer progression"**
*Authors: Wang Y, et al.*
摘要：通过免疫组化分析发现，EPS8L3在结直肠癌组织中高表达，并与患者预后不良相关。体外实验表明，重组EPS8L3蛋白可促进癌细胞侵袭，提示其作为治疗靶点的潜力。
3. **"EPS8L3 interacts with Rab5 to regulate endosomal trafficking"**
*Authors: Gonzalez S, et al.*
摘要：利用免疫共沉淀和活细胞成像技术，证实EPS8L3通过结合Rab5 GTPase调控内体运输过程，为理解其在胞吞作用中的分子机制提供了新证据。
4. **"Knockdown of EPS8L3 impairs neuronal development in vitro"**
*Authors: Patel R, et al.*
摘要：通过siRNA沉默EPS8L3表达，发现其缺失导致神经元突触生长受阻，表明EPS8L3在神经发育中可能通过调节肌动蛋白动力学发挥作用。
(注：以上文献为模拟生成，实际研究中请通过PubMed、Web of Science等数据库检索真实文献。)
The human EPS8L3 (Epidermal Growth Factor Receptor Pathway Substrate 8-like 3) protein belongs to the EPS8 family of cytoskeletal regulatory proteins involved in actin dynamics and cellular signaling. Encoded by the EPS8L3 gene, this ubiquitously expressed protein contains conserved functional domains, including an SH3 domain that mediates protein-protein interactions and a sterile alpha motif (SAM) implicated in subcellular localization. Unlike its paralogs (EPS8 and EPS8L2), EPS8L3 lacks a phosphotyrosine-binding (PTB) domain, suggesting functional divergence. It participates in regulating actin cytoskeleton remodeling, endocytosis, and receptor tyrosine kinase signaling pathways, particularly influencing cell motility, morphogenesis, and intracellular trafficking.
Recombinant human EPS8L3 protein is typically produced using prokaryotic or eukaryotic expression systems, enabling biochemical and functional studies. Its purification often involves affinity chromatography tags (e.g., GST, His-tag) to facilitate isolation. Research highlights its role in neurological development, cancer progression, and ciliogenesis, with dysregulation linked to neurodevelopmental disorders and metastasis. Recombinant forms are crucial for structural analysis, antibody development, and mechanistic investigations into EPS8L3's interaction networks. Recent studies also explore its potential as a therapeutic target, particularly in contexts where actin dynamics or EGFR/RAS signaling pathways are pathologically altered. Ongoing work aims to elucidate isoform-specific functions and disease-related molecular mechanisms.
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