| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EPS8 |
| Uniprot No | Q12929 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-822aa |
| 氨基酸序列 | MNGHISNHPSSFGMYPSQMNGYGSSPTFSQTDREHGSKTSAKALYEQRKNYARDSVSSVSDISQYRVEHLTTFVLDRKDAMITVDDGIRKLKLLDAKGKVWTQDMILQVDDRAVSLIDLESKNELENFPLNTIQHCQAVMHSCSYDSVLALVCKEPTQNKPDLHLFQCDEVKANLISEDIESAISDSKGGKQKRRPDALRMISNADPSIPPPPRAPAPAPPGTVTQVDVRSRVAAWSAWAADQGDFEKPRQYHEQEETPEMMAARIDRDVQILNHILDDIEFFITKLQKAAEAFSELSKRKKNKKGKRKGPGEGVLTLRAKPPPPDEFLDCFQKFKHGFNLLAKLKSHIQNPSAADLVHFLFTPLNMVVQATGGPELASSVLSPLLNKDTIDFLNYTVNGDERQLWMSLGGTWMKARAEWPKEQFIPPYVPRFRNGWEPPMLNFMGATMEQDLYQLAESVANVAEHQRKQEIKRLSTEHSSVSEYHPADGYAFSSNIYTRGSHLDQGEAAVAFKPTSNRHIDRNYEPLKTQPKKYAKSKYDFVARNNSELSVLKDDILEILDDRKQWWKVRNASGDSGFVPNNILDIVRPPESGLGRADPPYTHTIQKQRMEYGPRPADTPPAPSPPPTPAPVPVPLPPSTPAPVPVSKVPANITRQNSSSSDSGGSIVRDSQRHKQLPVDRRKSQMEEVQDELIHRLTIGRSAAQKKFHVPRQNVPVINITYDSTPEDVKTWLQSKGFNPVTVNSLGVLNGAQLFSLNKDELRTVCPEGARVYSQITVQKAALEDSSGSSELQEIMRRRQEKISAAASDSGVESFDEGSSH |
| 分子量 | 118.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人EPS8蛋白的3篇参考文献示例：
1. **文献名称**: *"Eps8 mediates cytoskeletal reorganization and metastatic progression in cancer"*
**作者**: Scita G., et al.
**摘要**: 该研究揭示了重组人EPS8蛋白通过调控肌动蛋白骨架重组促进肿瘤细胞迁移和侵袭的分子机制。实验表明EPS8与Abi1/Sos1复合物相互作用，激活Rac信号通路，从而驱动癌细胞转移。
2. **文献名称**: *"Biochemical characterization of recombinant human Eps8: A role in EGFR endocytosis"*
**作者**: Disanza A., et al.
**摘要**: 本研究通过大肠杆菌系统表达了重组人EPS8蛋白，并解析其结构域功能。结果表明EPS8的C端区域对表皮生长因子受体(EGFR)的内吞过程至关重要，调控受体下游信号传导的时效性。
3. **文献名称**: *"Structural insights into the actin-binding function of Eps8"*
**作者**: Hertzog M., et al.
**摘要**: 该研究利用重组EPS8蛋白的晶体结构分析，阐明了其结合肌动蛋白微丝的关键位点，并验证了EPS8在细胞伪足形成中的作用。突变实验显示其肌动蛋白成束活性依赖特定的螺旋结构域。
(注：上述文献为示例性质，实际引用时请核实具体文献信息。)
Epidermal growth factor receptor pathway substrate 8 (EPS8) is a multifunctional protein involved in regulating actin cytoskeleton dynamics, receptor signaling, and vesicular trafficking. Initially identified as a substrate of the epidermal growth factor receptor (EGFR), EPS8 interacts with signaling molecules like Rac and Rab5. influencing cellular processes such as migration, endocytosis, and membrane remodeling. Its modular structure, comprising a PTB domain, SH3-binding motifs, and an actin-binding region, enables diverse protein interactions. Dysregulation of EPS8 is linked to cancer progression, neurological disorders, and immune dysfunction, with overexpression observed in tumors, correlating with metastasis and poor prognosis.
Recombinant human EPS8 (rhEPS8) is produced via heterologous expression systems (e.g., E. coli or mammalian cells) for functional studies and therapeutic exploration. Purified rhEPS8 facilitates in vitro analyses of its molecular mechanisms, including actin capping/bundling activities and pathway modulation. Researchers utilize it to probe EPS8’s role in EGFR-mediated signaling cascades, neuronal development, or immune cell activation. Recent studies highlight its potential as a biomarker or therapeutic target, particularly in cancers reliant on EGFR or cytoskeletal dysregulation. rhEPS8 also aids drug discovery by enabling high-throughput screening for inhibitors disrupting EPS8-associated oncogenic networks. Its applications extend to structural biology, helping resolve atomic-level interactions critical for rational drug design.
×
