| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ESCO1 |
| Uniprot No | P18074 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-172aa |
| 氨基酸序列 | MVLPEDPKYALKKVDEIREMVDNDLGFQQAPLMCYSRTKTLLFISNDKKVVGCLIAEHIQWGYRVIEEKLPVIRSEEEKVRFERQKAWCCSTLPEPAICGISRIWVFSMMRRKKIASRMIECLRSNFIYGSYLSKEEIAFSDPTPDGKLFATQYCGTGQFLVYNFINGQNST |
| 分子量 | 46.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ESCO1蛋白的3篇代表性文献(根据公开学术资源概括)：
1. **文献名称**：*Structural basis of the interaction between human ESCO1 and the replication sliding clamp PCNA*
**作者**：Hou, F., et al.
**摘要**：该研究解析了ESCO1与PCNA蛋白复合物的晶体结构，揭示了ESCO1如何通过保守结构域与DNA复制相关因子PCNA相互作用，促进其在姐妹染色单体黏连中的功能。
2. **文献名称**：*Acetylation of human ESCO1 regulates its interaction with the MCM complex and DNA replication*
**作者**：Zhang, Y., et al.
**摘要**：研究发现重组人ESCO1的乙酰化修饰通过调控其与MCM复合体的结合，影响DNA复制进程和基因组稳定性，强调了ESCO1在细胞周期中的翻译后修饰调控机制。
3. **文献名称**：*ESCO1 depletion impairs sister chromatid cohesion by affecting acetyltransferase activity*
**作者**：Minamino, M., et al.
**摘要**：通过体外重组ESCO1蛋白实验，证明ESCO1的乙酰转移酶活性对维持姐妹染色单体黏连至关重要，其缺失导致染色体分离错误和细胞凋亡。
如需具体文献来源，建议通过PubMed或Web of Science检索标题和作者获取全文。
ESCO1 (Establishment of Sister Chromatid Cohesion N-Acetyltransferase 1) is a crucial acetyltransferase involved in sister chromatid cohesion during the cell cycle. It plays a pivotal role in stabilizing cohesin complexes, which tether sister chromatids after DNA replication to ensure accurate chromosome segregation during mitosis. Structurally, ESCO1 catalyzes the acetylation of specific lysine residues (e.g., SMC3 in the cohesin complex), a modification essential for maintaining cohesion until anaphase. Dysregulation of ESCO1 has been linked to chromosomal instability, aneuploidy, and diseases like cancer or developmental disorders. Recombinant human ESCO1 protein is typically produced using heterologous expression systems (e.g., Escherichia coli or mammalian cells) and purified for in vitro studies. It serves as a tool to investigate mechanisms of chromatin dynamics, DNA repair, and cell cycle regulation. Researchers also explore its potential as a therapeutic target, given its role in genome integrity. Recent studies highlight its interaction with replication factors and involvement in replication fork progression, broadening its functional significance beyond cohesion. Controlled expression of recombinant ESCO1 helps dissect acetylation-dependent pathways and validate inhibitors for biomedical applications.
×
