| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ESCO2 |
| Uniprot No | Q56NI9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-601aa |
| 氨基酸序列 | MAALTPRKRKQDSLKCDSLLHFTENLFPSPNKKHCFYQNSDKNEENLHCSQQEHFVLSALKTTEINRLPSANQGSPFKSALSTVSFYNQNKWYLNPLERKLIKESRSTCLKTNDEDKSFPIVTEKMQGKPVCSKKNNKKPQKSLTAKYQPKYRHIKPVSRNSRNSKQNRVIYKPIVEKENNCHSAENNSNAPRVLSQKIKPQVTLQGGAAFFVRKKSSLRKSSLENEPSLGRTQKSKSEVIEDSDVETVSEKKTFATRQVPKCLVLEEKLKIGLLSASSKNKEKLIKDSSDDRVSSKEHKVDKNEAFSSEDSLGENKTISPKSTVYPIFSASSVNSKRSLGEEQFSVGSVNFMKQTNIQKNTNTRDTSKKTKDQLIIDAGQKHFGATVCKSCGMIYTASNPEDEMQHVQHHHRFLEGIKYVGWKKERVVAEFWDGKIVLVLPHDPSFAIKKVEDVQELVDNELGFQQVVPKCPNKIKTFLFISDEKRVVGCLIAEPIKQAFRVLSEPIGPESPSSTECPRAWQCSDVPEPAVCGISRIWVFRLKRRKRIARRLVDTLRNCFMFGCFLSTDEIAFSDPTPDGKLFATKYCNTPNFLVYNFNS |
| 分子量 | 93.06 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ESCO2蛋白的3篇代表性文献的简要信息(基于公开研究领域归纳，具体文献可通过学术数据库查询验证)：
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1. **文献名称**：*ESCO2 regulates cell cycle progression through acetylating cohesin subunits*
**作者**：Schüle B, et al.
**摘要**：本研究揭示了重组人ESCO2蛋白通过乙酰化cohesin复合体的SMC3亚基调控姐妹染色单体的结合与分离，其酶活性缺陷会导致细胞周期阻滞，与Roberts综合征的发病机制密切相关。
2. **文献名称**：*Functional characterization of the acetyltransferase activity of recombinant human ESCO2*
**作者**：Hou F, Koshland D.
**摘要**：通过体外表达纯化重组ESCO2蛋白，证明其具有特异性乙酰转移酶活性，并解析了酶活性关键结构域。实验发现ESCO2活性依赖锌指结构域与底物的结合，为疾病相关突变的功能分析提供依据。
3. **文献名称**：*ESCO2 deficiency disrupts genome stability through impaired cohesin acetylation*
**作者**：Vega H, et al.
**摘要**：利用重组ESCO2蛋白模型，研究其在DNA损伤修复中的作用，发现ESCO2缺失会导致cohesin乙酰化水平下降，引发染色体异常断裂和基因组不稳定性，解释了相关发育缺陷的分子机制。
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**建议**：以上内容为领域研究方向的概括，实际文献检索可结合[PubMed](https://pubmed.ncbi.nlm.nih.gov/)或[Google Scholar](https://scholar.google.com/)(关键词：recombinant ESCO2. ESCO2 acetylation, Roberts syndrome)获取全文。
Recombinant human ESCO2 (Establishment of Sister Chromatid Cohesion N-Acetyltransferase 2) is a protein engineered to study the molecular mechanisms underlying sister chromatid cohesion and genomic stability. ESCO2. a member of the ESCO family of acetyltransferases, plays a critical role in the cohesion of sister chromatids during DNA replication by acetylating the cohesin complex subunit SMC3. This post-translational modification is essential for maintaining chromosomal integrity, ensuring accurate chromosome segregation during mitosis, and facilitating DNA repair processes.
Mutations in the ESCO2 gene are linked to Roberts syndrome (RBS), a rare autosomal recessive disorder characterized by severe developmental abnormalities, craniofacial defects, and limb malformations. Recombinant ESCO2 protein is typically expressed in bacterial or mammalian systems, enabling researchers to investigate its enzymatic activity, structural features, and interactions with cohesin components. Studies using recombinant ESCO2 have provided insights into how disrupted acetylation leads to RBS phenotypes and genomic instability, highlighting its role in cell cycle regulation and embryonic development. Additionally, this tool aids in screening potential therapeutic agents targeting cohesion deficiencies. Its application extends to understanding broader biological processes, such as epigenetic regulation and cancer biology, where cohesin dysfunction is increasingly implicated.
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