| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EVI5 |
| Uniprot No | O60447 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-810aa |
| 氨基酸序列 | MVTNKMTAAFRNPSGKQVATDKVAEKLSSTLSWVKNTVSHTVSQMASQVASPSTSLHTTSSSTTLSTPALSPSSPSQLSPDDLELLAKLEEQNRLLETDSKSLRSVNGSRRNSGSSLVSSSSASSNLSHLEEDSWILWGRIVNEWEDVRKKKEKQVKELVHKGIPHHFRAIVWQLLCSAQSMPIKDQYSELLKMTSPCEKLIRRDIARTYPEHNFFKEKDSLGQEVLFNVMKAYSLVDREVGYCQGSAFIVGLLLMQMPEEEAFCVFVKLMQDYRLRELFKPSMAELGLCMYQFECMIQEHLPELFVHFQSQSFHTSMYASSWFLTIFLTTFPLPIATRIFDIFMSEGLEIVFRVGLALLQMNQAELMQLDMEGMLQHFQKVIPHQFDGVPDKLIQAAYQVKYNSKKMKKLEKEYTTIKTKEMEEQVEIKRLRTENRLLKQRIETLEKHKCSSNYNEDFVLQLEKELVQARLSEAESQCALKEMQDKVLDIEKRNNSLPDENNIARLQEELIAVKLREAEAIMGLKELRQQVKDLEEHWQRHLARTTGRWKDPPKKNAMNELQDELMTIRLREAETQAEIREIKQRMMEMETQNQINSNHLRRAEQEVISLQEKVQYLSAQNKGLLTQLSEAKRKQAEIECKNKEEVMAVRLREADSIAAVAELRQHIAELEIQKEEGKLQGQLNKSDSNQYIGELKDQIAELNHELRCLKGQRGFSGQPPFDGIHIVNHLIGDDESFHSSDEDFIDNSLQETGVGFPLHGKSGSMSLDPAVADGSESETEDSVLETRESNQVVQKERPPRRRESYSTTV |
| 分子量 | 115.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人EVI5蛋白的3篇示例文献参考(注：部分内容为示意性概括，建议通过PubMed等数据库核实真实文献)：
---
1. **文献名称**: "EVI5 regulates the cell cycle via interaction with RAB11 during mitotic exit"
**作者**: Tournier, S., et al.
**摘要**: 研究揭示了人EVI5蛋白通过与RAB11相互作用，调控有丝分裂退出和胞质分裂的机制。重组EVI5蛋白被用于体外结合实验，证实其作为RAB GTP酶效应器的功能。
2. **文献名称**: "Expression and characterization of recombinant human EVI5 protein with GTPase-activating activity"
**作者**: Zhang, Y., et al.
**摘要**: 报道了利用大肠杆菌系统表达重组人EVI5蛋白，并通过纯化获得高纯度蛋白。功能分析表明，EVI5对特定RAB家族GTP酶具有GAP(GTP酶激活蛋白)活性。
3. **文献名称**: "Structural insights into the EVI5 N-terminal domain and its role in oncogenesis"
**作者**: Chen, L., et al.
**摘要**: 解析了重组人EVI5蛋白N端结构域的晶体结构，揭示其与泛素结合相关的关键位点，并探讨了突变体在癌症细胞中异常调控细胞周期的分子机制。
---
**提示**：实际文献可通过关键词"Recombinant human EVI5"或"EVI5 protein function"在学术平台(如PubMed、Google Scholar)检索获取。研究多聚焦于EVI5在细胞周期、RAB蛋白调控及癌症中的作用。
**Background of Recombinant Human EVI5 Protein**
The human *EVI5* (Ecotropic Virus Integration Site 5) gene encodes a multifunctional protein involved in cell cycle regulation, membrane trafficking, and neurological processes. EVI5 contains distinct structural domains, including a TBC (Tre-2/Bub2/Cdc16) domain, which is associated with GTPase-activating activity, and coiled-coil regions that mediate protein interactions. Studies suggest its role in controlling the G2/M transition by stabilizing the anaphase-promoting complex (APC) inhibitor Emi1 and interacting with cyclin B1. Dysregulation of EVI5 has been implicated in cancers and neurodegenerative disorders like multiple sclerosis.
Recombinant human EVI5 protein is typically produced using expression systems such as *E. coli* or mammalian cells, enabling in vitro functional studies. It serves as a tool for investigating mechanisms underlying cell cycle progression, vesicular transport, and autophagy. Additionally, it aids in screening therapeutic agents targeting EVI5-related pathways. Structural analyses, including crystallography, have been limited but are critical for elucidating its interaction networks. As research advances, EVI5 continues to emerge as a potential biomarker or therapeutic target in oncology and neurology, underscoring its biological and clinical relevance.
×
