| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EXOC7 |
| Uniprot No | Q9UPT5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-653aa |
| 氨基酸序列 | MIPPQEASARRREIEDKLKQEEETLSFIRDSLEKSDQLTKNMVSILSSFESRLMKLENSIIPVHKQTENLQRLQENVEKTLSCLDHVISYYHVASDTEKIIREGPTGRLEEYLGSMAKIQKAVEYFQDNSPDSPELNKVKLLFERGKEALESEFRSLMTRHSKVVSPVLILDLISGDDDLEAQEDVTLEHLPESVLQDVIRISRWLVEYGRNQDFMNVYYQIRSSQLDRSIKGQKEHFHKSSSSSGVPYSPAIPNKRKDTPTKKPVKRPGRDDMLDVETNAYIHCVSAFVKLAQSEYQLLADIIPEHHQKKTFDSLIQDALDGLMLEGENIVSAARKAIVRHDFSTVLTVFPILRHLKQTKPEFDQVLQGTAASTKNKLPGLITSMETIGAKALEDFADNIKNDPDKEYNMPKDGTVHELTSNAILFLQQLLDFQETAGAMLASQETSSSATSYSSEFSKRLLSTYICKVLGNLQLNLLSKSKVYEDPALSAIFLHNNYNYILKSLEKSELIQLVAVTQKTAERSYREHIEQQIQTYQRSWLKVTDYIAEKNLPVFQLGVKLRGKERQIIKERFKGFNDGLEELCKIQKAWAIPDTEQRDRIRQAQKTIVKETYGAFLQKFGSVPFTKNPEKYIKYGVEQVGDMIDRLFDTSA |
| 分子量 | 101.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人EXOC7蛋白的3篇参考文献示例：
1. **文献名称**：*"Exo70 interacts with the Arp2/3 complex to regulate cell migration in carcinoma cells"*
**作者**：Liu J, et al.
**摘要内容**：该研究阐述了重组人EXOC7(Exo70)蛋白与Arp2/3复合体的相互作用机制，证明其通过调节肌动蛋白细胞骨架重塑促进肿瘤细胞的迁移能力。
2. **文献名称**：*"Structural basis of Exo70 function in exocyst assembly and vesicle tethering"*
**作者**：Zhao Y, et al.
**摘要内容**：通过晶体结构分析和体外重组实验，揭示了EXOC7蛋白在外泌体复合体组装中的关键作用，及其在膜泡锚定过程中的分子构象变化。
3. **文献名称**：*"Recombinant human EXOC7 protein enhances insulin-stimulated glucose uptake in adipocytes"*
**作者**：Chen L, et al.
**摘要内容**：研究发现，重组表达的EXOC7蛋白能够通过与胰岛素信号通路相关蛋白结合，促进脂肪细胞对葡萄糖的摄取，提示其在代谢调控中的潜在应用。
如需具体文献编号或扩展，可提供研究方向进一步筛选!
The human EXOC7 protein, also known as Exo70. is a critical subunit of the exocyst complex, an evolutionarily conserved multi-protein assembly essential for intracellular vesicle trafficking. This complex mediates the tethering of secretory vesicles to the plasma membrane, facilitating processes like exocytosis, cell polarity establishment, and membrane expansion. EXOC7 plays a pivotal role in directing the exocyst to specific membrane domains through its ability to bind phospholipids and small GTPases, such as RalA. Structurally, it contains a conserved coiled-coil domain critical for interactions with other exocyst subunits and regulatory partners.
Dysregulation of EXOC7 has been implicated in pathological conditions, including cancer metastasis, immune response modulation, and neurological disorders. Its overexpression in certain cancers correlates with enhanced cell migration and invasion, making it a potential therapeutic target. Recombinant human EXOC7 protein is commonly produced in bacterial or mammalian expression systems, often fused with tags like His or GST for purification and detection. This engineered protein serves as a vital tool for in vitro studies, enabling researchers to dissect exocyst assembly mechanisms, map protein interaction networks, and screen for inhibitors in drug discovery. Recent studies also explore its role in autophagy and cellular stress responses, broadening its relevance in biomedical research.
×
