| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EXOC3L4 |
| Uniprot No | Q17RC7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-722aa |
| 氨基酸序列 | MPSPQTDTPGPELQSPKEAEEPQTPAQGSRRTSSRKEPNAHRKDGTRLGLGSLRQAFSRASQRALTQVSKEDTGLFRRSSCSLFRSFRQALNDGPATGHSQATPEVPSGVMNGVSQQASTGAASEELKPEAEGKSVADLITERQLLAAFEQLLRLETLLVAEKASRTFEQDPTAFARRAMDVCLLYDGLAAEIGAIVRETLDSDGVDAAALAELARVVSAEEEAHPSPPDDGDFLRTPRRWRQHWEEAVRRSAQERVRRPGAGWAFGEAEGASGLAQLLAELGGLVRRDLQKVRQEVQPAYAAAGFPAWEVYLRAFHSAVAQRLQELARDARGCEQLYILLDWAANVYGSPDFLGAPGLALPAEPLPPLLAPDVWARLESDYTSFLEAKIASCFDSILQLEQSHWAAAEVPEVLQGLYQAPLSMDVHMLVAEHVKAAGAISAELEATTLRICTRALGLFVPRFEKAFLASEAVSEPHLGAYINACEELRTSLLSRFPGTQEELEKPLVTATCSFQKHLLQGLQRELQPLFRVVCTRDWLTQDWLHPLMDKVVTFAGHLQRVARPRAQETLQEVHRFVVREYLARALRPRERFRGMERMHGSQKMSLDAQAISDTFQGLGSEATWLDQAIQCVAEILGETYKDDIQRHLETLIRSYPDIRRDHILAILALRRLGRRRNQHLLQHTEDLLRAAAGAAGAEAPRGRVLFEEIKVPSAMAVLITCV |
| 分子量 | 79.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人EXOC3L4蛋白的3-4篇虚构参考文献示例(文献信息为模拟内容，实际引用请核实真实来源)：
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1. **文献名称**: "Overexpression of Recombinant EXOC3L4 Promotes Hepatocellular Carcinoma Metastasis via Exosome-Mediated Signaling"
**作者**: Zhang, Y. et al.
**摘要**: 本研究构建了重组人EXOC3L4蛋白的真核表达系统，发现其过表达通过增强外泌体释放，激活MAPK通路，促进肝癌细胞迁移和体内转移，提示EXOC3L4可能成为肝癌治疗的潜在靶点。
2. **文献名称**: "A Novel Bacterial Expression System for Human EXOC3L4 and Its Role in Vesicle Trafficking"
**作者**: Lee, S. et al.
**摘要**: 开发了一种高效原核表达体系，纯化重组EXOC3L4并解析其晶体结构。功能实验表明，该蛋白通过与Exocyst复合体其他亚基相互作用，调控高尔基体到细胞膜的囊泡运输。
3. **文献名称**: "EXOC3L4 Knockdown Impairs Autophagy in Colorectal Cancer by Disrupting RAB11 Localization"
**作者**: Wang, L. et al.
**摘要**: 利用重组EXOC3L4蛋白及siRNA技术，揭示其在结直肠癌细胞中通过结合RAB11调控自噬体形成，缺失导致自噬障碍并抑制肿瘤生长，为靶向癌症代谢提供新思路。
4. **文献名称**: "EXOC3L4 Dysregulation in Alzheimer's Disease: Insights from Recombinant Protein-Based Neuronal Models"
**作者**: Smith, J. & Patel, R.
**摘要**: 通过重组EXOC3L4处理神经元细胞，发现其异常表达导致β-淀粉样蛋白分泌增加，并损害突触可塑性，提示Exocyst复合体失调可能参与阿尔茨海默病病理进程。
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注：以上文献为示例性内容，实际研究中请通过PubMed、Google Scholar等平台检索真实发表的论文。如需进一步协助查找真实文献，请提供更多研究背景或关键词。
EXOC3L4 (Exocyst Complex Component 3 Like 4) is a member of the exocyst complex family, which comprises eight subunits involved in regulating vesicle trafficking and exocytosis. This protein shares structural homology with EXOC3. a core exocyst component mediating polarized secretion and membrane expansion. However, EXOC3L4’s specific biological functions remain less characterized. It is encoded by a gene located on human chromosome 4 and is ubiquitously expressed across tissues, with notable upregulation observed in certain cancers, including hepatocellular carcinoma and glioma, suggesting potential roles in oncogenesis.
Recombinant human EXOC3L4 protein is engineered for in vitro studies to elucidate its molecular interactions, cellular localization, and involvement in exocytosis or membrane repair pathways. Produced typically in mammalian or insect expression systems, it retains post-translational modifications critical for functional analyses. Researchers employ this protein to investigate its binding partners, regulatory mechanisms, and possible association with cancer progression via in vitro binding assays, pull-down experiments, or structural studies. Emerging evidence hints at its interaction with small GTPases and cytoskeletal elements, implicating it in tumor cell migration and invasion. Further exploration of recombinant EXOC3L4 may advance understanding of exocyst complex diversification, vesicle transport dynamics, and its therapeutic potential as a biomarker or drug target.
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