| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EVX1 |
| Uniprot No | P49640 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-407aa |
| 氨基酸序列 | MESRKDMVVFLDGGQLGTLVGKRVSNLSEAVGSPLPEPPEKMVPRGCLSPRAVPPATRERGGGGPEEEPVDGLAGSAAGPGAEPQVAGAAMLGPGPPAPSVDSLSGQGQPSSSDTESDFYEEIEVSCTPDCATGNAEYQHSKGSGSEALVGSPNGGSETPKSNGGSGGGGSQGTLACSASDQMRRYRTAFTREQIARLEKEFYRENYVSRPRRCELAAALNLPETTIKVWFQNRRMKDKRQRLAMTWPHPADPAFYTYMMSHAAAAGGLPYPFPSHLPLPYYSPVGLGAASAASAAASPFSGSLRPLDTFRVLSQPYPRPELLCAFRHPPLYPGPAHGLGASAGGPCSCLACHSGPANGLAPRAAAASDFTCASTSRSDSFLTFAPSVLSKASSVALDQREEVPLTR |
| 分子量 | 71.17 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是模拟的3条关于重组人EVX1蛋白的参考文献示例(仅供参考，实际文献需通过学术数据库验证)：
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1. **文献名称**：*Expression and Purification of Recombinant Human EVX1 Protein in a Prokaryotic System*
**作者**：Smith A, et al.
**摘要**：本研究成功构建了人EVX1基因的原核表达载体，利用大肠杆菌系统表达并纯化具有生物活性的重组EVX1蛋白，优化了表达条件以提高蛋白产量，为后续功能研究提供基础材料。
2. **文献名称**：*Functional Role of Recombinant EVX1 in Regulating Embryonic Stem Cell Differentiation*
**作者**：Lee H, Kim S.
**摘要**：通过体外实验证明，重组人EVX1蛋白可特异性结合靶基因启动子区域，激活或抑制下游信号通路，显著影响胚胎干细胞的定向分化，提示其在早期发育中的关键调控作用。
3. **文献名称**：*Structural Analysis of Recombinant EVX1 Protein and Its DNA-Binding Domain*
**作者**：Wang Y, et al.
**摘要**：采用X射线晶体学解析重组人EVX1蛋白的三维结构，明确其DNA结合域的关键氨基酸残基，为研究EVX1与靶基因相互作用机制提供结构基础。
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**提示**：以上内容为示例性模拟，实际文献需通过PubMed、Web of Science等平台检索。建议以关键词“recombinant human EVX1”“EVX1 protein purification”或“EVX1 functional study”进行精确查询。
Recombinant human EVX1 (Even-skipped homeobox 1) protein is a transcription factor encoded by the EVX1 gene, a member of the homeobox gene family critical in embryonic development. Homeobox genes regulate axial patterning, cell differentiation, and tissue morphogenesis by binding DNA via conserved homeodomains. EVX1. specifically, plays a role in mesoderm formation, posterior embryo development, and limb bud patterning during early vertebrate embryogenesis. It is also implicated in regulating pluripotency and differentiation in stem cells.
The recombinant form of EVX1 is typically produced using prokaryotic (e.g., E. coli) or eukaryotic expression systems to ensure proper folding and post-translational modifications. This enables functional studies in vitro or in vivo, bypassing challenges associated with endogenous protein isolation. Researchers utilize recombinant EVX1 to investigate its molecular mechanisms in developmental pathways, epigenetic regulation, and interactions with signaling molecules like Wnt or BMP. Notably, aberrant EVX1 expression has been linked to cancers, including gastric and colorectal malignancies, where it may act as a tumor suppressor or oncogene depending on context.
Recent applications include its use in regenerative medicine models to modulate cell fate and in high-throughput screens for drug discovery. Studies also explore its role in reactivating developmental programs for tissue repair. However, its pleiotropic effects and context-dependent activity require careful validation in experimental settings. The availability of recombinant EVX1 facilitates structural analyses, antibody production, and pathway mapping, advancing both basic research and therapeutic development.
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