关键信息
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基因名
ARHGEF5
- 应用
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别名
ARHG5_HUMAN; ARHGEF 5; ARHGEF5; DKFZp686N1969; ephexin 3
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q12774
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表达区间
1-519aa
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氨基酸序列
MGGFSRRCSKLINSSQLLYQEYSDVVLNKEIQSQQRLESLSETPGPSSPRQPRKALVSSESYLQRLSMASSGSLWQEIPVVRNSTVLLSMTHEDQKLQEVKFELIVSEASYLRSLNIAVDHFQLSTSLRATLSNQEHQWLFSRLQDVRDVSATFLSDLEENFENNIFSFQVCDVVLNHAPDFRRVYLPYVTNQTYQERTFQSLMNSNSNFREVLEKLESDPVCQRLSLKSFLILPFQRITRLKLLLQNILKRTQPGSSEEAEATKAHHALEQLIRDCNNNVQSMRRTEELIYLSQKIEFECKIFPLISQSRWLVKSGELTALEFSASPGLRRKLNTRPVHLHLFNDCLLLSRPREGSRFLVFDHAPFSSIRGEKCEMKLHGPHKNLFRLFLRQNTQGAQAEFLFRTETQSEKLRWISALAMPREELDLLECYNSPQVQCLRAYKPRENDELALEKADVVMVTQQSSDGWLEGVRLSDGERGWFPVQQVEFISNPEVRAQNLKEAHRVKTAKLQLVEQQA
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分子量
82.83 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
ARHGEF5, also known as p115 RhoGEF, is a member of the Rho guanine nucleotide exchange factor (GEF) family, which plays a crucial role in the regulation of Rho GTPases, key molecular switches that control various cellular processes including cytoskeletal dynamics, cell adhesion, and migration. The dysregulation of Rho GEFs, including ARHGEF5, has been implicated in various diseases, notably cancer, as they influence tumor cell proliferation, invasion, and metastasis. Research into ARHGEF5 has revealed its involvement in signaling pathways related to cell growth and stress response, making it a potential target for therapeutic intervention. Additionally, ARHGEF5 has been linked to cardiovascular diseases and neurodegenerative disorders, underscoring its multifunctional roles in different biological contexts. The production and characterization of recombinant ARHGEF5 protein is essential for elucidating its structure and function, as well as for exploring its interactions with other cellular partners. Understanding the molecular mechanisms by which ARHGEF5 operates may provide insights into its role in disease pathology and highlight novel strategies for drug development. Thus, the study of ARHGEF5 holds significant promise for advancing our knowledge of Rho GTPase signaling and its implications in health and disease.
