| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EXOC5 |
| Uniprot No | O00471 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-708aa |
| 氨基酸序列 | ATTAELFEE PFVADEYIER LVWRTPGGGS RGGPEAFDPK RLLEEFVNHI QELQIMDERI QRKVEKLEQQ CQKEAKEFAK KVQELQKSNQ VAFQHFQELD EHISYVATKV CHLGDQLEGV NTPRQRAVEA QKLMKYFNEF LDGELKSDVF TNSEKIKEAA DIIQKLHLIA QELPFDRFSE VKSKIASKYH DLECQLIQEF TSAQRRGEIS RMREVAAVLL HFKGYSHCVD VYIKQCQEGA YLRNDIFEDA GILCQRVNKQ VGDIFSNPET VLAKLIQNVF EIKLQSFVKE QLEECRKSDA EQYLKNLYDL YTRTTNLSSK LMEFNLGTDK QTFLSKLIKS IFISYLENYI EVETGYLKSR SAMILQRYYD SKNHQKRSIG TGGIQDLKER IRQRTNLPLG PSIDTHGETF LSQEVVVNLL QETKQAFERC HRLSDPSDLP RNAFRIFTIL VEFLCIEHID YALETGLAGI PSSDSRNANL YFLDVVQQAN TIFHLFDKQF NDHLMPLISS SPKLSECLQK KKEIIEQMEM KLDTGIDRTL NCMIGQMKHI LAAEQKKTDF KPEDENNVLI QYTNACVKVC AYVRKQVEKI KNSMDGKNVD TVLMELGVRF HRLIYEHLQQ YSYSCMGGML AICDVAEYRK CAKDFKIPMV LHLFDTLHAL CNLLVVAPDN LKQVCSGEQL ANLDKNILHS FVQLRADYRS ARLARHFS |
| 分子量 | 81.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人EXOC5蛋白的模拟参考文献示例(实际文献需通过学术数据库检索)：
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1. **文献名称**：*Structural Insights into Human EXOC5 Subunit and Its Role in Exocyst Assembly*
**作者**：Chen L, et al.
**摘要**：本研究解析了重组人EXOC5蛋白的晶体结构，揭示了其与外泌体复合体其他亚基(如EXOC6和EXOC8)的相互作用机制，表明EXOC5在维持复合体稳定性及囊泡运输中的关键作用。
2. **文献名称**：*EXOC5 Knockdown Impairs Cell Migration via Dysregulation of EGFR Recycling*
**作者**：Wang Y, et al.
**摘要**：通过构建重组EXOC5缺陷型细胞模型，发现EXOC5通过调控EGFR的回收通路影响细胞迁移，提示其在癌症转移中的潜在治疗靶点价值。
3. **文献名称**：*Recombinant EXOC5 Protein Attenuates Neurodegenerative Pathology in Alzheimer’s Disease Models*
**作者**：Kim S, et al.
**摘要**：利用重组人EXOC5蛋白干预阿尔茨海默病细胞模型，发现其能减少β-淀粉样蛋白沉积并改善神经元突触功能，可能与调节外泌体介导的毒性蛋白清除有关。
4. **文献名称**：*Optimization of Recombinant Human EXOC5 Expression in E. coli for Functional Studies*
**作者**：Zhang R, et al.
**摘要**：报道了一种高效的大肠杆菌表达系统，用于生产具有生物活性的重组EXOC5蛋白，并通过体外实验验证其与外泌体复合体的结合能力。
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**提示**：以上内容为示例，实际文献建议通过PubMed、Google Scholar等平台检索关键词(如“recombinant human EXOC5”、“EXOC5 function”)。如需具体论文，可提供更详细的研究方向以便进一步协助!
Recombinant human EXOC5 protein is a biologically engineered form of the exocyst complex component 5. a critical subunit of the evolutionarily conserved exocyst complex. The exocyst, an octameric protein complex, regulates intracellular vesicle trafficking and tethering to the plasma membrane, facilitating processes like exocytosis, cell polarization, and membrane expansion. EXOC5 (also known as Sec10) plays a pivotal role in maintaining complex structural integrity and mediating interactions with other exocyst subunits (e.g., Sec3. Sec5) and downstream effectors. It contains conserved domains for binding Rab GTPases and phosphoinositides, enabling spatiotemporal control of secretory vesicle docking. Dysregulation of EXOC5 is implicated in cancer metastasis, ciliopathies, and neurodevelopmental disorders.
The recombinant protein, typically expressed in mammalian or insect cell systems with affinity tags (e.g., His-, GST-), retains native conformation and function for *in vitro* studies. It serves as a tool to investigate exocyst assembly, membrane trafficking mechanisms, and protein-protein interactions via pull-down assays, structural analysis, or functional reconstitution. Recent studies utilize recombinant EXOC5 to explore its role in autophagy, cell migration, and receptor recycling, offering potential therapeutic insights for exocyst-related pathologies. Current research also focuses on mapping its phosphorylation sites and regulatory networks in response to extracellular cues.
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