| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EXOSC10 |
| Uniprot No | Q01780 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-885aa |
| 氨基酸序列 | MAPPSTREPRVLSATSATKSDGEMVLPGFPDADSFVKFALGSVVAVTKASGGLPQFGDEYDFYRSFPGFQAFCETQGDRLLQCMSRVMQYHGCRSNIKDRSKVTELEDKFDLLVDANDVILERVGILLDEASGVNKNQQPVLPAGLQVPKTVVSSWNRKAAEYGKKAKSETFRLLHAKNIIRPQLKFREKIDNSNTPFLPKIFIKPNAQKPLPQALSKERRERPQDRPEDLDVPPALADFIHQQRTQQVEQDMFAHPYQYELNHFTPADAVLQKPQPQLYRPIEETPCHFISSLDELVELNEKLLNCQEFAVDLEHHSYRSFLGLTCLMQISTRTEDFIIDTLELRSDMYILNESLTDPAIVKVFHGADSDIEWLQKDFGLYVVNMFDTHQAARLLNLGRHSLDHLLKLYCNVDSNKQYQLADWRIRPLPEEMLSYARDDTHYLLYIYDKMRLEMWERGNGQPVQLQVVWQRSRDICLKKFIKPIFTDESYLELYRKQKKHLNTQQLTAFQLLFAWRDKTARREDESYGYVLPNHMMLKIAEELPKEPQGIIACCNPVPPLVRQQINEMHLLIQQAREMPLLKSEVAAGVKKSGPLPSAERLENVLFGPHDCSHAPPDGYPIIPTSGSVPVQKQASLFPDEKEDNLLGTTCLIATAVITLFNEPSAEDSKKGPLTVAQKKAQNIMESFENPFRMFLPSLGHRAPVSQAAKFDPSTKIYEISNRWKLAQVQVQKDSKEAVKKKAAEQTAAREQAKEACKAAAEQAISVRQQVVLENAAKKRERATSDPRTTEQKQEKKRLKISKKPKDPEPPEKEFTPYDYSQSDFKAFAGNSKSKVSSQFDPNKQTPSGKKCIAAKKIKQSVGNKSMSFPTGKSDRGFRYNWPQR |
| 分子量 | 127.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人EXOSC10蛋白的3篇参考文献示例(注：部分内容为假设性概括，实际文献需核实)：
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1. **文献名称**: "Structural and functional analysis of the human exosome subunit EXOSC10"
**作者**: Schmidt, C., et al.
**摘要**: 研究通过重组表达和纯化人EXOSC10蛋白，结合X射线晶体学揭示了其3D结构，并验证其在外切体复合体中的3’→5’核糖核酸外切酶活性，强调了关键结构域在RNA降解中的作用。
2. **文献名称**: "EXOSC10 modulates mRNA stability and promotes cancer metastasis via interaction with oncogenic transcripts"
**作者**: Li, Y., et al.
**摘要**: 利用重组人EXOSC10蛋白进行体外RNA结合实验，发现其通过选择性降解肿瘤抑制性mRNA促进结直肠癌转移，揭示了EXOSC10的靶向调控机制。
3. **文献名称**: "Dysregulation of EXOSC10 disrupts ribosome biogenesis and causes neurodegeneration in a Drosophila model"
**作者**: Park, J., & Kim, S.
**摘要**: 研究通过在果蝇中表达重组人EXOSC10蛋白，发现其功能缺失导致rRNA加工异常及神经元死亡，表明EXOSC10缺陷与神经退行性疾病的关联。
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**备注**：以上文献名称与摘要均为示例，实际参考文献需通过PubMed、Google Scholar等平台以“recombinant human EXOSC10”“EXOSC10 function”等关键词检索获取。EXOSC10(亦称RRP6)相关研究多集中于RNA代谢、癌症及神经疾病领域，重组蛋白技术常被用于解析其生化功能或病理机制。
Human EXOSC10 (Exosome Component 10), also known as RRP6. is a critical subunit of the evolutionarily conserved RNA exosome complex, a multi-protein machinery essential for RNA processing, surveillance, and degradation. The exosome plays central roles in 3'→5' RNA turnover, ribosome biogenesis, and quality control of non-coding RNAs. EXOSC10 is a nuclear-specific catalytic component endowed with 3'→5' exoribonuclease activity, distinguishing it from other non-catalytic exosome subunits. It contains an N-terminal PIN domain and a C-terminal HRDC domain, facilitating interactions with RNA and exosome cofactors.
Recombinant human EXOSC10 protein is engineered via heterologous expression systems (e.g., *E. coli* or mammalian cells) to study its enzymatic functions, structural properties, and role in RNA metabolism. Its recombinant form enables in vitro analysis of RNA-binding kinetics, exonuclease activity, and interactions with cofactors like MTR4 or C1D. Research links EXOSC10 to diseases, including neurodegeneration (e.g., spinal muscular atrophy) and cancer, where dysregulated RNA processing drives pathogenesis. Studies using recombinant EXOSC10 have also explored its involvement in viral RNA degradation and stress granule dynamics. As a tool, it aids in dissecting exosome-mediated RNA surveillance mechanisms and screening for modulators targeting RNA decay pathways.
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