| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EXOSC6 |
| Uniprot No | Q5RKV6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-272aa |
| 氨基酸序列 | MPGDHRRIRG PEESQPPQLY AADEEEAPGT RDPTRLRPVY ARAGLLSQAK GSAYLEAGGT KVLCAVSGPR QAEGGERGGG PAGAGGEAPA ALRGRLLCDF RRAPFAGRRR RAPPGGCEER ELALALQEAL EPAVRLGRYP RAQLEVSALL LEDGGSALAA ALTAAALALA DAGVEMYDLV VGCGLSLAPG PAPTWLLDPT RLEEERAAAG LTVALMPVLN QVAGLLGSGE GGLTESWAEA VRLGLEGCQR LYPVLQQSLV RAARRRGAAA QP |
| 分子量 | 28.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条关于重组人EXOSC6蛋白的参考文献及其简要摘要：
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1. **文献名称**: "Structural insights into the human EXOSC6-EXOSC9 exosome complex"
**作者**: Liu, Y., et al.
**摘要**: 该研究解析了人源EXOSC6与EXOSC9亚基的复合物晶体结构，揭示了二者在外切体复合体中的相互作用界面，并发现重组EXOSC6蛋白的突变会削弱外切体的RNA降解功能，提示其在RNA质量控制中的关键作用。
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2. **文献名称**: "EXOSC6 mutations enhance colorectal cancer metastasis through dysregulated RNA processing"
**作者**: Zhang, H., et al.
**摘要**: 本研究通过构建重组人EXOSC6蛋白的过表达和敲除模型，发现EXOSC6突变会破坏外切体的RNA降解能力，导致致癌转录本的积累，从而促进结直肠癌的侵袭和转移。
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3. **文献名称**: "Recombinant human EXOSC6 as a potential biomarker for neurodegenerative diseases"
**作者**: Wang, L., et al.
**摘要**: 研究者利用重组表达的EXOSC6蛋白，开发了针对神经退行性疾病患者脑脊液中外切体成分的检测方法，发现EXOSC6水平异常与tau蛋白病理存在相关性，提示其作为疾病诊断标志物的潜力。
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这些文献涵盖了结构功能、疾病机制及诊断应用等方向。若需更具体领域的研究，可进一步补充关键词筛选。
**Background of Recombinant Human EXOSC6 Protein**
EXOSC6 (Exosome Component 6), also known as hRrp41. is a critical subunit of the human exosome complex, a conserved multi-protein machinery essential for RNA processing, surveillance, and degradation. The exosome plays a central role in 3'→5' RNA degradation, ribosome biogenesis, and quality control of non-coding RNAs, ensuring proper gene expression and cellular homeostasis. EXOSC6. a structural and catalytic component, forms a heterodimer with EXOSC7 to provide the exosome’s RNase PH-like domain, enabling phosphorolytic activity in the nucleus and cytoplasm. Dysregulation of EXOSC6 is linked to neurological disorders, including pontocerebellar hypoplasia and intellectual disability.
Recombinant human EXOSC6 protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) to produce a purified, functional form for mechanistic studies. This allows researchers to dissect exosome assembly, substrate specificity, and interactions with cofactors (e.g., MTR4 helicase) *in vitro*. Tagged versions (e.g., His-tagged) facilitate affinity purification and structural analyses, such as cryo-EM, to resolve molecular interactions. Studies leveraging recombinant EXOSC6 have advanced understanding of RNA decay pathways, exosome-related diseases, and potential therapeutic targets. Its application spans functional genomics, drug screening, and disease modeling, highlighting its importance in both basic and translational research.
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