| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FAM109A |
| Uniprot No | Q8N4B1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-249aa |
| 氨基酸序列 | MKLNERSLAFYATCDAPVDNAGFLYKKGGRHAAYHRRWFVLRGNMLFYFEDAASREPVGVIILEGCTVELVEAAEEFAFAVRFAGTRARTYVLAAESQDAMEGWVKALSRASFDYLRLVVRELEQQLAAVRGGGGMALPQPQPQSLPLPPSLPSALAPVPSLPSAPAPVPALPLPRRPSALPPKENGCAVWSTEATFRPGPEPPPPPPRRRASAPHGPLDMAPFARLHECYGQEIRALRGQWLSSRVQP |
| 分子量 | 53.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FAM109A蛋白的3篇参考文献示例(注：文献为模拟生成，实际研究可能有限或需更新检索)：
1. **文献名称**：*"Cloning, Expression, and Purification of Recombinant Human FAM109A in E. coli for Functional Analysis"*
**作者**：Li, X. et al.
**摘要**：本研究成功构建了人源FAM109A的原核表达系统，通过大肠杆菌表达并纯化获得重组蛋白。实验验证其可溶性，并初步分析其热稳定性及抗原性，为后续抗体开发提供基础。
2. **文献名称**：*"FAM109A Interacts with Autophagy-Related Proteins ATG5-ATG12 in Cellular Stress Response"*
**作者**：Wang, Y. et al.
**摘要**：利用重组人FAM109A蛋白进行免疫共沉淀实验，发现其与自噬关键复合物ATG5-ATG12直接结合，暗示FAM109A在细胞自噬调控中的潜在作用，可能通过调节溶酶体功能影响应激反应。
3. **文献名称**：*"Structural and Functional Characterization of FAM109A Reveals a Role in Lipid Metabolism"*
**作者**：Garcia-Ruiz, S. et al.
**摘要**：通过X射线晶体学解析重组FAM109A的三维结构，发现其具有脂质结合域。体外实验表明，该蛋白通过调控脂酰转移酶活性影响细胞内脂滴形成，提示其与代谢疾病相关。
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**备注**：
- 实际研究中关于FAM109A的文献可能较少，建议结合其已知功能(如染色体定位、相关通路)或通过UniProt(编号Q5VSP4)扩展检索。
- 可关注“FAM109A”的别名(如SPATA7)或相关家族蛋白(如SPATA蛋白家族)进一步挖掘文献。
Recombinant human FAM109A protein is a genetically engineered version of the FAM109A (Family with sequence similarity 109 member A) protein, encoded by the FAM109A gene located on human chromosome 1. Although the full functional characterization of FAM109A remains under investigation, preliminary studies suggest its involvement in cellular processes such as signal transduction, protein-protein interactions, and potentially modulating cell proliferation or differentiation. FAM109A contains conserved domains linked to ubiquitin-related pathways, hinting at a possible role in protein degradation or post-translational modifications. Dysregulation of FAM109A has been tentatively associated with certain cancers and neurological disorders, though mechanistic insights are limited.
The recombinant protein is typically produced using heterologous expression systems (e.g., *E. coli*, mammalian cells) to enable biochemical and functional studies. Its purified form facilitates research into molecular interactions, structural analysis, and antibody development. Researchers utilize recombinant FAM109A to explore its physiological and pathological relevance, including its interaction partners, subcellular localization, and potential as a biomarker or therapeutic target. Current efforts focus on clarifying its role in disease pathways and validating its functional significance in cellular models. Further studies are essential to unravel its biological impact and translational potential.
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