| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FAM110A |
| Uniprot No | Q9BQ89 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-295aa |
| 氨基酸序列 | MPVHTLSPGAPSAPALPCRLRTRVPGYLLRGPADGGARKPSAVERLEADKAKYVKSLHVANTRQEPVQPLLSKQPLFSPETRRTVLTPSRRALPGPCRRPQLDLDILSSLIDLCDSPVSPAEASRTPGRAEGAGRPPPATPPRPPPSTSAVRRVDVRPLPASPARPCPSPGPAAASSPARPPGLQRSKSDLSERFSRAAADLERFFNFCGLDPEEARGLGVAHLARASSDIVSLAGPSAGPGSSEGGCSRRSSVTVEERARERVPYGVSVVERNARVIKWLYGLRQARESPAAEG |
| 分子量 | 57.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与重组人FAM110A蛋白相关的参考文献示例(注：内容为假设性示例，实际文献需根据真实研究补充)：
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**1. 文献名称**："FAM110A promotes mitotic spindle formation by interacting with microtubules"
**作者**：Chen L, et al.
**摘要**：该研究通过重组人FAM110A蛋白表达及免疫共沉淀实验，发现其直接结合微管并参与有丝分裂纺锤体组装，抑制FAM110A会导致细胞周期停滞于G2/M期，提示其在细胞分裂中的关键作用。
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**2. 文献名称**："Recombinant FAM110A inhibits hepatocellular carcinoma proliferation via PI3K/AKT pathway"
**作者**：Wang Y, et al.
**摘要**：利用原核系统表达重组人FAM110A蛋白，发现其在肝癌细胞中通过下调PI3K/AKT信号通路抑制肿瘤生长，并通过体外实验证实其对迁移和侵袭的调控作用。
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**3. 文献名称**："Structural characterization of human FAM110A and its centrosomal localization"
**作者**：Smith J, et al.
**摘要**：采用重组FAM110A蛋白进行X射线晶体学分析，解析其N端结构域的二聚化特性，并验证其在中心体的定位，揭示其与细胞周期调控蛋白CEP192的相互作用。
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**4. 文献名称**："FAM110A deficiency causes premature senescence via oxidative stress"
**作者**：Kim S, et al.
**摘要**：通过CRISPR敲低和重组蛋白回补实验，证明FAM110A缺失导致ROS累积和细胞早衰，补充重组蛋白可逆转该表型，表明其通过抗氧化途径维持基因组稳定性。
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(注：以上内容仅为学术示例，真实文献需通过PubMed、Web of Science等平台检索。)
**Background of Recombinant Human FAM110A Protein**
Recombinant human FAM110A is a genetically engineered protein belonging to the FAM110 (family with sequence similarity 110) family, which is conserved across species and implicated in cellular processes like mitosis and cell cycle regulation. FAM110A is localized to the centrosome and nucleus, playing roles in microtubule organization, spindle assembly, and maintaining genomic stability during cell division. Studies suggest its involvement in modulating cell proliferation and survival, potentially linked to cancer progression due to dysregulated expression in certain tumors.
Structurally, FAM110A contains conserved coiled-coil domains, facilitating protein-protein interactions. Its recombinant form is typically produced in *E. coli* or mammalian expression systems, enabling functional studies in vitro or in cell-based models. Purification tags (e.g., His-tag) are often added for isolation. Researchers utilize this protein to explore its molecular mechanisms, including interactions with cell cycle regulators (e.g., Aurora kinases) and signaling pathways. Additionally, it serves as an antigen for antibody development or a tool in high-throughput screens targeting mitosis-related disorders. Understanding FAM110A’s role could offer insights into mitotic errors and therapeutic strategies for cancers or developmental diseases. Further research is needed to fully elucidate its physiological and pathological functions.
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