| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FAM71E1 |
| Uniprot No | Q6IPT2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-231aa |
| 氨基酸序列 | MGPPLWPDLQEPPPPGTSSQIRSPLLCDVIKPAPHHDVTVRVVPPPRFLPLLLRPLPSDGDIAMRRDRGPKPALGGAGEVEPGGMAASPTGRPRRLQRYLQSGEFDQFRDFPIFESNFVQVTRLGEVANEVTMGVAASSPALELPDLLLLAGPAKENGHLQLFGLFPLKFVQLFVHDKSRCQLEVKLNTSRTFYLQLRAPLKTRDREFGQWVRLLYRLRFLSASAVPFTQE |
| 分子量 | 52.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FAM71E1蛋白的模拟参考文献示例(实际研究中请通过数据库核实具体文献)：
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1. **文献名称**: *"FAM71E1 is a critical regulator of sperm flagellar structure in humans"*
**作者**: Smith J, Li X, et al.
**摘要**: 本研究通过重组表达人FAM71E1蛋白，发现其在小鼠精子鞭毛形成中具有重要作用。敲除FAM71E1会导致精子活力下降和鞭毛结构异常，提示其在男性不育中的潜在机制。
2. **文献名称**: *"Expression and functional characterization of recombinant human FAM71E1 in cellular models"*
**作者**: Zhang Y, Wang Q, et al.
**摘要**: 利用哺乳动物细胞系统成功表达并纯化重组FAM71E1蛋白，发现其具有磷酸酶活性，可能通过调控细胞内信号通路影响生殖细胞发育。
3. **文献名称**: *"Proteomic analysis of FAM71E1 interactions reveals its role in microtubule dynamics"*
**作者**: Gupta R, Kim S, et al.
**摘要**: 通过重组FAM71E1蛋白的亲和层析实验，鉴定出与微管相关蛋白的相互作用，提示其参与细胞分裂和细胞骨架重塑过程。
4. **文献名称**: *"Structural insights into FAM71E1 by recombinant protein crystallography"*
**作者**: Tanaka M, et al.
**摘要**: 首次解析重组人FAM71E1蛋白的晶体结构，揭示其N端结构域具有独特的折叠模式，为设计靶向该蛋白的分子探针提供基础。
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**注意事项**：
- 以上文献为基于FAM71E1相关研究方向的模拟示例，实际引用需查询PubMed、Web of Science等数据库。
- 推荐检索关键词："recombinant FAM71E1", "FAM71E1 protein function", "FAM71 family proteins"。
Here’s a concise overview of recombinant human FAM71E1 protein:
**Background:**
FAM71E1 (Family with Sequence Similarity 71 Member E1) is a poorly characterized protein encoded by the *FAM71E1* gene in humans. It belongs to the FAM71 family, which comprises proteins with conserved domains but largely undefined biological roles. FAM71E1 is primarily expressed in reproductive tissues, particularly the testis, suggesting a potential role in spermatogenesis or sperm function. Structural predictions indicate it may contain disordered regions and phosphorylation sites, hinting at regulatory or signaling functions. However, its exact mechanism and physiological significance remain unclear.
**Recombinant Production:**
Recombinant human FAM71E1 protein is typically generated using heterologous expression systems (e.g., *E. coli* or mammalian cells) to enable functional studies. The recombinant form allows researchers to investigate its biochemical properties, interactions, and potential involvement in cellular pathways. Tags like His-tags are often added for purification via affinity chromatography.
**Research Context:**
Current studies focus on linking FAM71E1 to male infertility, as mutations or dysregulation in testis-specific genes often correlate with reproductive disorders. Preliminary evidence suggests its localization in sperm components, possibly influencing motility or fertilization. However, further validation is needed to establish its role in health and disease. Recombinant FAM71E1 serves as a critical tool for developing antibodies, structural studies, and unraveling its molecular functions.
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