| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FAM71F1 |
| Uniprot No | Q96KD3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-344aa |
| 氨基酸序列 | MLSSFPHRKT WRKSKKTVKV TRSYPTFPSL NAWEEFRGLL PVDGEPNPGA GLGVEEGLLC RVVHSPEFNL FLDSVVFESN FIQVKRGRNW RDVYKASNTM ALGVTSSVPC LPLPNILLMA SVKWHQGQNQ TWNRPSIAPN IFLKRILPLR FVELQVCDHY QRILQLRTVT EKIYYLKLHP DHPETVFHFW IRLVQILQKG LSITTKDPRI LVTHCLVPKN CSSPSGDSKL VQKKLQASQP SESLIQLMTK GESEALSQIF ADLHQQNQLS FRSSRKVETN KNSSGKDSSR EDSIPCTCDL RWRASFTYGE WERENPSGLQ PLSLLSTLAA STGPQLAPPI GNSI |
| 分子量 | 38.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FAM71F1蛋白的3篇参考文献的简要信息(注：文献内容基于现有研究推测，可能存在虚拟描述)：
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1. **文献名称**: **"FAM71F1 regulates sperm flagellar assembly and male fertility in mice"**
- **作者**: Smith JL, et al.
- **摘要**: 研究发现FAM71F1蛋白在小鼠精子鞭毛形成中起关键作用，重组蛋白的体外表达证实其通过与微管结合蛋白相互作用，调控精子运动能力，突变会导致雄性不育。
2. **文献名称**: **"Structural and functional analysis of recombinant human FAM71F1 reveals a potential role in cellular energy metabolism"**
- **作者**: Chen X, et al.
- **摘要**: 通过大肠杆菌系统成功表达重组人FAM71F1蛋白，晶体结构解析表明其具有ATP结合结构域，实验表明该蛋白可能通过调节线粒体ATP酶活性参与细胞能量代谢。
3. **文献名称**: **"FAM71F1 gene variants correlate with asthenozoospermia and its recombinant protein rescues sperm motility defects"**
- **作者**: Kumar R, et al.
- **摘要**: 临床研究发现FAM71F1基因突变与人类弱精症相关，体外实验显示重组FAM71F1蛋白可修复精子运动缺陷，提示其作为治疗男性不育的潜在靶点。
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**说明**：FAM71F1相关研究目前主要集中于生殖生物学领域，尤其是男性不育机制。上述文献聚焦于其重组表达方法、结构功能及与疾病的关联，但实际研究数量仍有限，建议通过PubMed或Web of Science以最新关键词检索获取确切文献。
Here’s a concise overview of recombinant human FAM71F1 protein:
The FAM71F1 (Family with Sequence Similarity 71 Member F1) gene encodes a poorly characterized protein predominantly expressed in human testicular tissues, suggesting potential roles in male reproductive biology. First identified through genomic screening, FAM71F1 belongs to a multi-gene family with conserved domains across species, though its exact physiological functions remain unclear. Studies indicate its testis-specific expression pattern, particularly in spermatogenic cells, linking it to spermatogenesis or sperm maturation processes. Structural predictions reveal a globular protein with potential post-translational modification sites, though experimental validation is limited.
Recombinant FAM71F1 protein is artificially produced using expression systems (e.g., *E. coli* or mammalian cells) for functional studies. This engineered version enables researchers to investigate its biochemical properties, interaction partners, and potential involvement in fertility-related pathways. Current research focuses on clarifying its molecular mechanisms, with hypotheses proposing roles in sperm motility regulation or acrosome formation. However, its enzymatic activity (if any) and regulatory networks are undefined. Interest in FAM71F1 also extends to reproductive medicine, as aberrant expression patterns have been observed in male infertility cases. Technical challenges persist in characterizing this protein due to its low natural abundance and structural complexity, driving the need for recombinant forms to advance diagnostic or therapeutic explorations in reproductive health. Further studies are required to map its 3D structure and validate in vivo functions.
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