| 纯度 | > 90 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | CCDC104 |
| Uniprot No | Q96G28 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-342aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMAAEEED EVEWVVESIA GFLRGPDWSI PILDFVEQKC EVFDDEEESK LTYTEIHQEY KELVEKLLEG YLKEIGINED QFQEACTSPL AKTHTSQAIL QPVLAAEDFT IFKAMMVQKN IEMQLQAIRI IQERNGVLPD CLTDGSDVVS DLEHEEMKIL REVLRKSKEE YDQEEERKRK KQLSEAKTEE PTVHSSEAAI MNNSQGDGEH FAHPPSEVKM HFANQSIEPL GRKVERSETS SLPQKDLKIP GLEHASIEGP IANLSVLGTE ELRQREHYLK QKRDKLMSMR KDMRTKQIQN MEQKGKPTGE VEEMTEKPEM TAEEKQTLLK RRLLAEKLKE EVINK |
| 预测分子量 | 42 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CCDC104重组蛋白的3篇参考文献及其摘要概括(注：文献为虚拟示例，实际需根据真实数据库检索补充)：
1. **文献名称**：*"Expression and functional characterization of recombinant CCDC104 in mitotic regulation"*
**作者**：Zhang L, et al.
**摘要**：本研究成功在大肠杆菌中表达并纯化了CCDC104重组蛋白，证实其通过与微管蛋白相互作用参与细胞有丝分裂调控，并发现其缺失导致染色体排列异常。
2. **文献名称**：*"Structural analysis of CCDC104 reveals a novel coiled-coil domain essential for nuclear localization"*
**作者**：Li X, Wang Y, et al.
**摘要**：通过X射线晶体学解析了CCDC104重组蛋白的N端结构，发现其卷曲螺旋结构域对核定位信号(NLS)的结合至关重要，为研究其核转运机制提供结构基础。
3. **文献名称**：*"CCDC104 interacts with p53: Implications in cancer cell apoptosis"*
**作者**：Chen R, et al.
**摘要**：利用重组CCDC104蛋白进行免疫共沉淀实验，发现其与肿瘤抑制蛋白p53直接互作，并通过调控p53稳定性影响癌细胞凋亡通路。
4. **文献名称**：*"High-throughput screening of CCDC104-binding compounds using recombinant protein-based assays"*
**作者**：Wang H, et al.
**摘要**：基于CCDC104重组蛋白建立高通量药物筛选平台，筛选出多个潜在小分子抑制剂，为靶向CCDC104的疾病治疗提供候选化合物。
(注：以上文献信息为示例，实际引用需根据具体研究补充真实发表文献。)
**Background of CCDC104 Recombinant Protein**
CCDC104 (Coiled-Coil Domain Containing 104) is a protein encoded by the *CCDC104* gene, which is conserved across vertebrates and implicated in cellular processes such as cytoskeletal organization, cell division, and intracellular trafficking. The protein is characterized by its coiled-coil domains, structural motifs that facilitate protein-protein interactions, enabling its potential role in scaffolding or regulatory complexes. While the full functional spectrum of CCDC104 remains under investigation, studies suggest its involvement in mitosis and microtubule dynamics, possibly through interactions with components of the mitotic spindle or centrosomal proteins.
Recombinant CDC104 protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cell lines) to produce purified, biologically active forms for functional studies. Its recombinant version often includes affinity tags (e.g., His-tag) to streamline purification. Structural analyses, including X-ray crystallography or cryo-EM, aim to elucidate its 3D conformation and interaction interfaces.
Research on CCDC104 has gained traction due to its potential links to human diseases. For instance, dysregulation of cytoskeletal proteins is associated with cancer progression, ciliopathies, and neurodevelopmental disorders. CCDC104’s interaction with microtubule-associated proteins (e.g., TUBG1) hints at roles in maintaining genomic stability, making it a candidate for exploring mechanisms of chromosomal segregation errors or drug resistance in malignancies.
Despite progress, many aspects of CCDC104 remain enigmatic, including its precise molecular partners, post-translational modifications, and tissue-specific functions. Current studies leverage recombinant CCDC104 to dissect its biochemical properties, subcellular localization, and contributions to cellular pathways, offering insights into its physiological and pathological relevance. This protein thus represents a promising target for both basic research and therapeutic exploration.
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