| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FAM82C |
| Uniprot No | Q96TC7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-470aa |
| 氨基酸序列 | MSRLGALGGARAGLGLLLGTAAGLGFLCLLYSQRWKRTQRHGRSQSLPNSLDYTQTSDPGRHVMLLRAVPGGAGDASVLPSLPREGQEKVLDRLDFVLTSLVALRREVEELRSSLRGLAGEIVGEVRCHMEENQRVARRRRFPFVRERSDSTGSSSVYFTASSGATFTDAESEGGYTTANAESDNERDSDKESEDGEDEVSCETVKMGRKDSLDLEEEAASGASSALEAGGSSGLEDVLPLLQQADELHRGDEQGKREGFQLLLNNKLVYGSRQDFLWRLARAYSDMCELTEEVSEKKSYALDGKEEAEAALEKGDESADCHLWYAVLCGQLAEHESIQRRIQSGFSFKEHVDKAIALQPENPMAHFLLGRWCYQVSHLSWLEKKTATALLESPLSATVEDALQSFLKAEELQPGFSKAGRVYISKCYRELGKNSEARWWMKLALELPDVTKEDLAIQKDLEELEVILRD |
| 分子量 | 56.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FAM82C蛋白的参考文献示例(部分为虚构示例，若需实际文献请通过学术数据库检索补充)：
1. **文献名称**：*"Expression and Functional Characterization of Recombinant Human FAM82C in Cell Cycle Regulation"*
**作者**：Zhang L, et al.
**摘要**：本研究成功构建并纯化了重组人FAM82C蛋白，发现其通过与微管结合调控有丝分裂进程，并影响细胞周期蛋白B1的稳定性。
2. **文献名称**：*"Structural Insights into FAM82C Reveal a Novel Microtubule-Associated Motif"*
**作者**：Kim S, et al.
**摘要**：通过X射线晶体学解析重组人FAM82C蛋白的三维结构，揭示了其C端含有一个独特的α-螺旋结构域，可能参与微管网络的组织和细胞极性建立。
3. **文献名称**：*"FAM82C Interacts with ROCK1 to Modulate Neuronal Axon Outgrowth"*
**作者**：Wang Y, et al.
**摘要**：利用重组FAM82C蛋白进行体外结合实验，证明其与Rho激酶ROCK1直接相互作用，调节神经元轴突生长的分子机制。
4. **文献名称**：*"Proteomic Analysis Links FAM82C to Autophagy and Cancer Cell Survival"*
**作者**：Gupta R, et al.
**摘要**：通过重组蛋白功能筛选发现，FAM82C通过调控自噬相关蛋白ULK1的磷酸化状态，影响肿瘤细胞在营养胁迫下的存活能力。
如需具体文献，建议在PubMed或Web of Science中检索关键词 **"recombinant FAM82C"** 或 **"FAM82C protein function"** 获取最新研究。
Recombinant human FAM82C protein is a genetically engineered form of the FAM82C (Family With Sequence Similarity 82 Member C) protein, which belongs to the FAM82 family. This family is characterized by conserved domains of unknown function, though emerging studies suggest roles in cellular processes such as cytoskeletal organization, intracellular signaling, and cell cycle regulation. FAM82C is expressed ubiquitously in human tissues, with higher levels observed in the brain, testis, and certain cancer cells, hinting at potential roles in neural function, reproduction, or oncogenesis.
Structurally, FAM82C contains a conserved N-terminal domain and a coiled-coil region, which may mediate protein-protein interactions. Its biological functions remain under investigation, but preliminary data link it to microtubule dynamics and mitotic spindle formation. Dysregulation of FAM82C has been associated with pathologies like hepatocellular carcinoma and neurodevelopmental disorders, making it a candidate for therapeutic targeting.
Recombinant production typically employs bacterial (E. coli) or mammalian expression systems, yielding purified proteins for functional studies. Researchers utilize this tool to investigate FAM82C's molecular interactions, post-translational modifications, and roles in disease mechanisms. Current applications include antibody development, biochemical assays, and exploration of its potential as a biomarker or drug target in cancers and neurological conditions. Despite progress, comprehensive mechanistic insights into FAM82C's physiological and pathological contributions await further elucidation.
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