| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OAS2 |
| Uniprot No | P29728-2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-687aa |
| 氨基酸序列 | MGNGESQLSSVPAQKLGWFIQEYLKPYEECQTLIDEMVNTICDVLQEPEQ FPLVQGVAIGGSYGRKTVLRGNSDGTLVLFFSDLKQFQDQKRSQRDILDK TGDKLKFCLFTKWLKNNFEIQKSLDGFTIQVFTKNQRISFEVLAAFNALS LNDNPSPWIYRELKRSLDKTNASPGEFAVCFTELQQKFFDNRPGKLKDLI LLIKHWHQQCQKKIKDLPSLSPYALELLTVYAWEQGCRKDNFDIAEGVRT VLELIKCQEKLCIYWMVNYNFEDETIRNILLHQLQSARPVILDPVDPTNN VSGDKICWQWLKKEAQTWLTSPNLDNELPAPSWNVLPAPLFTTPGHLLDK FIKEFLQPNKCFLEQIDSAVNIIRTFLKENCFRQSTAKIQIVRGGSTAKG TALKTGSDADLVVFHNSLKSYTSQKNERHKIVKEIHEQLKAFWREKEEEL EVSFEPPKWKAPRVLSFSLKSKVLNESVSFDVLPAFNALGQLSSGSTPSP EVYAGLIDLYKSSDLPGGEFSTCFTVLQRNFIRSRPTKLKDLIRLVKHWY KECERKLKPKGSLPPKYALELLTIYAWEQGSGVPDFDTAEGFRTVLELVT QYQQLCIFWKVNYNFEDETVRKFLLSQLQKTRPVILDPAEPTGDVGGGDR WCWHLLAKEAKEWLSSPCFKDGTGNPIPPWKVPVKVI |
| 预测分子量 | 105 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条关于 **OAS2重组蛋白** 的参考文献示例(注：文献为假设性示例，实际引用需核实)：
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1. **《Recombinant OAS2 Protein Expression and Its Role in Antiviral Response》**
- **作者**: Hartman, R. et al.
- **摘要**: 研究在大肠杆菌系统中表达并纯化重组OAS2蛋白，验证其通过合成2'-5'寡腺苷酸激活RNase L的酶活性，证实其在抑制RNA病毒复制中的关键作用。
2. **《Structural Analysis of Human OAS2 Reveals Substrate Binding Specificity》**
- **作者**: Li, X. & Chen, Z.
- **摘要**: 通过X射线晶体学解析OAS2重组蛋白的催化结构域三维结构，揭示其与ATP/双链RNA的相互作用机制，为设计靶向抗病毒药物提供结构基础。
3. **《OAS2 Recombinant Protein Modulates Interferon Signaling in Cancer Cells》**
- **作者**: Smith, J. et al.
- **摘要**: 利用哺乳动物细胞表达系统生成功能性OAS2重组蛋白，证明其在乳腺癌细胞中通过干扰素通路抑制肿瘤生长，并增强化疗敏感性。
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如需具体文献，建议通过 **PubMed/Google Scholar** 搜索关键词：*"OAS2 recombinant protein" + expression/function/structure*。
Oligoadenylate synthetase 2 (OAS2) is a key enzyme in the innate immune response, belonging to the OAS family of proteins that play a critical role in antiviral defense. Primarily induced by type I interferons during viral infection, OAS2 is activated upon sensing cytoplasmic double-stranded RNA (dsRNA), a molecular signature of many viruses. Structurally, it contains conserved nucleotidyltransferase domains that catalyze the synthesis of 2′-5′-linked oligoadenylates (2-5A) from ATP. These 2-5A molecules act as secondary messengers to activate latent ribonuclease L (RNase L), which degrades viral and cellular RNA, thereby limiting viral replication and spread. OAS2’s activity is tightly regulated, with variations in its expression or genetic polymorphisms linked to differences in susceptibility to viral infections, including hepatitis C, SARS-CoV-2. and other RNA viruses.
Recombinant OAS2 protein is produced using biotechnological platforms, such as bacterial or mammalian expression systems, to study its enzymatic properties, structure-function relationships, and interactions within antiviral pathways. Its recombinant form enables researchers to explore mechanisms of immune evasion by viruses, screen for antiviral compounds, or model host-pathogen interactions. Additionally, OAS2 has been implicated in non-antiviral processes, including cancer and autoimmune diseases, making its recombinant version a valuable tool for broader biomedical research. Studies using recombinant OAS2 have also contributed to understanding how single-nucleotide polymorphisms (e.g., rs1293767) affect enzymatic activity and disease outcomes. By providing a purified, functional protein, recombinant OAS2 accelerates both basic research and therapeutic development, such as designing agonists to enhance antiviral responses or biomarkers for disease prognosis.
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