| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NUP205 |
| Uniprot No | Q92621 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 全长 |
| 氨基酸序列 | full |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NUP205重组蛋白的3篇参考文献的简要总结：
---
1. **文献名称**：*Structural and functional analysis of the Nup82-Nup159-Nup205 complex in nuclear pore assembly*
**作者**：Hoelz A, et al.
**摘要**：该研究通过重组表达NUP205及其相互作用蛋白Nup82和Nup159.解析了其在核孔复合体(NPC)中的结构。实验表明，重组NUP205对维持NPC的稳定性和介导mRNA出核运输至关重要。
---
2. **文献名称**：*NUP205 depletion inhibits Wnt/β-catenin signaling by repressing Ran-mediated nuclear import*
**作者**：Zhang Y, et al.
**摘要**：利用重组NUP205蛋白进行体外结合实验，发现其通过调控Ran GTPase的核质转运影响Wnt/β-catenin信号通路。研究揭示了NUP205在癌症细胞增殖中的潜在作用。
---
3. **文献名称**：*A novel mutation in NUP205 causes autosomal recessive primary microcephaly*
**作者**：Shaheen R, et al.
**摘要**：通过重组突变型NUP205蛋白的功能分析，发现其异常导致核孔组装缺陷，阻碍神经细胞分裂。该研究首次将NUP205突变与人类小头症相关联。
---
4. **文献名称**：*Reconstitution of the human nuclear pore complex reveals a role for NUP205 in membrane curvature*
**作者**：Beck M, et al.
**摘要**：利用重组NUP205蛋白在体外重建核孔复合体，结合冷冻电镜技术，发现其通过诱导膜弯曲促进核孔的双层膜融合，为NPC组装机制提供了新见解。
---
这些研究通过重组NUP205蛋白，分别从结构、信号调控、疾病关联及组装机制角度揭示了其功能。如需具体文献链接或补充，可进一步提供数据库检索关键词。
NUP205 (Nucleoporin 205) is a critical component of the nuclear pore complex (NPC), a large protein assembly embedded in the nuclear envelope that regulates nucleocytoplasmic transport. As a scaffold nucleoporin, NUP205 plays a structural role in maintaining NPC architecture and stability. It belongs to the NUP107-160 subcomplex, which forms the NPC's structural framework and contributes to selective transport of macromolecules. Human NUP205 is a 205 kDa protein encoded by the *NUP205* gene, characterized by multiple α-solenoid domains that mediate protein-protein interactions within the NPC.
Research on NUP205 has revealed its involvement in diverse cellular processes beyond passive transport. It participates in mitotic spindle assembly, chromatin organization, and gene expression regulation. Mutations in *NUP205* are linked to developmental disorders and diseases, including primary ovarian insufficiency and certain cancers, highlighting its biological significance. However, studying endogenous NUP205 is challenging due to its large size, low cellular abundance, and tight association with other nucleoporins.
Recombinant NUP205 proteins, produced through heterologous expression systems (e.g., mammalian cells, bacteria, or insect cells), enable detailed biochemical and structural analyses. These engineered proteins often include affinity tags (e.g., His-tag) to facilitate purification. Researchers use recombinant NUP205 to investigate its interactions with transport receptors (e.g., importins/exportins), map binding domains, and study pathogenic mutations. Recent cryo-EM studies employing recombinant NUP205 have advanced understanding of NPC assembly and dynamics. Challenges remain in producing full-length functional protein due to its size (~1.800 amino acids) and complex post-translational modifications. Ongoing work focuses on developing truncated variants and co-expression strategies to overcome these limitations.
×
