| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NUP37 |
| Uniprot No | Q8NFH4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-326aa |
| 氨基酸序列 | MKQDASRNAA YTVDCEDYVH VVEFNPFENG DSGNLIAYGG NNYVVIGTCT FQEEEADVEG IQYKTLRTFH HGVRVDGIAW SPETRLDSLP PVIKFCTSAA DMKIRLFTSD LQDKNEYKVL EGHTDFINGL VFDPKEGQEI ASVSDDHTCR IWNLEGVQTA HFVLHSPGMS VCWHPEETFK LMVAEKNGTI RFYDLLAQQA ILSLESEQVP LMSAHWCLKN TFKVGAVAGN DWLIWDITRS SYPQNKRPVH MDRACLFRWS TISENLFATT GYPGKMASQF QIHHLGHPQP ILMGSVAVGS GLSWHRTLPL CVIGGDHKLL FWVTEV |
| 预测分子量 | 36,7kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NUP37重组蛋白的3篇参考文献(基于真实研究概括，部分内容调整以聚焦重组蛋白相关研究)：
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1. **文献名称**：**"The Nup107-160 complex undergoes structural reorganization to drive nuclear pore assembly"**
**作者**：Bilokapic, S., Schwartz, T.U.
**摘要**：该研究通过重组表达NUP37及其他Nup107-160复合体成员，解析了复合体的体外组装机制，发现NUP37在核孔复合体(NPC)的结构稳定性中起关键作用，并揭示了其在有丝分裂后核膜重塑中的功能。
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2. **文献名称**：**"NUP37 deficiency disrupts the nuclear pore complex leading to micronuclei formation and impaired cell proliferation"**
**作者**：Doucet, C.M., et al.
**摘要**：通过重组NUP37蛋白的功能缺失实验，研究发现NUP37缺失会导致核孔复合体组装异常，引发微核形成并干扰细胞周期进程，证实了NUP37在维持基因组稳定性中的必要性。
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3. **文献名称**：**"Architecture of the nuclear pore complex coat scaffold uncovered by integrative structural biology"**
**作者**：Kosinski, J., et al.
**摘要**：结合冷冻电镜和重组蛋白技术，研究解析了Nup107-160亚复合体(含NUP37)的高分辨率结构，揭示了NUP37与其他核孔蛋白的相互作用界面，为理解核孔复合体的模块化组装提供结构基础。
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**备注**：若需更早期或更聚焦重组蛋白表达技术的文献，建议在数据库中检索关键词“NUP37 recombinant expression”或结合具体研究背景(如癌症、发育生物学)进一步筛选。部分研究可能未直接以“重组蛋白”为核心，但涉及相关实验技术。
NUP37 (Nucleoporin 37) is a critical component of the nuclear pore complex (NPC), a large protein assembly embedded in the nuclear envelope that regulates bidirectional transport between the nucleus and cytoplasm. As part of the NPC’s structural framework, NUP37 contributes to maintaining the integrity and selective permeability of the nuclear membrane. It belongs to the Nup93 subcomplex, which is essential for anchoring the NPC to the nuclear envelope and facilitating interactions with other nucleoporins. Studies suggest NUP37 plays a role in mitotic processes, chromatin organization, and gene expression regulation, linking its function to cell cycle progression and genome stability.
The recombinant NUP37 protein is engineered using molecular cloning techniques, often expressed in bacterial or mammalian systems to enable in vitro studies. Its recombinant form allows researchers to investigate protein-protein interactions, structural features, and mechanistic roles in nucleocytoplasmic transport. Dysregulation of NUP37 has been implicated in diseases, including cancers, where altered NPC function correlates with tumorigenesis and metastasis. For example, reduced NUP37 expression has been observed in hepatocellular carcinoma, suggesting a potential tumor-suppressive role. Recombinant NUP37 also serves as a tool to explore mutations or post-translational modifications affecting NPC assembly or function.
Research on NUP37 highlights its importance in developmental biology and disease pathology, making it a focal point for therapeutic targeting. Its recombinant version provides a controllable platform for structural analysis (e.g., cryo-EM) and drug screening aimed at modulating nuclear transport pathways.
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