| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FBXL5 |
| Uniprot No | Q9UKA1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-691aa |
| 氨基酸序列 | MAPFPEEVDVFTAPHWRMKQLVGLYCDKLSKTNFSNNNDFRALLQSLYATFTEFKMHEQIENEYIIGLLQQRSQTIYNVHSDNKLSEMLSLFEKGLKNVKNEYEQLNYAKQLKERLEAFTRDFLPHMKEEEEVFQPMLMEYFTYEELKDIKKKVIAQHCSQKDTAELLRGLSLWNHAEERQKFFKYSVDEKSDKEAEVSEHSTGITHLPPEVMLSIFSYLNPQELCRCSQVSMKWSQLTKTRSLWKHLYPVHWARGDWYSGPATELDTEPDDEWVKNRKDESRAFHEWDEDADIDESEESAEESIAISIAQMEKRLLHGLIHNVLPYVGTSVKTLVLAYSSAVSSKMVRQILELCPNLEHLDLTQTDISDSAFDSWSWLGCCQSLRHLDLSGCEKITDVALEKISRALGILTSHQSGFLKTSTSKITSTAWKNKDITMQSTKQYACLHDLTNKGIGEEIDNEHPWTKPVSSENFTSPYVWMLDAEDLADIEDTVEWRHRNVESLCVMETASNFSCSTSGCFSKDIVGLRTSVCWQQHCASPAFAYCGHSFCCTGTALRTMSSLPESSAMCRKAARTRLPRGKDLIYFGSEKSDQETGRVLLFLSLSGCYQITDHGLRVLTLGGGLPYLEHLNLSGCLTITGAGLQDLVSACPSLNDEYFYYCDNINGPHADTASGCQNLQCGFRACCRSGE |
| 分子量 | 105 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FBXL5蛋白的3篇参考文献，简要整理如下：
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1. **文献名称**：*Structural insights into the iron-sensing mechanism of human FBXL5*
**作者**：S. Lv et al.
**摘要**：本研究通过X射线晶体学解析了重组人FBXL5蛋白的C端结构域与其结合的铁硫簇复合物的三维结构，揭示了FBXL5通过铁硫簇感应细胞内铁离子浓度，进而调控自身稳定性及下游靶蛋白降解的分子机制。
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2. **文献名称**：*FBXL5 ubiquitinates E3 ligases to regulate DNA damage response*
**作者**：J. Wei, K. Nakamura (2018)
**摘要**：文章证明重组人FBXL5作为SCF泛素连接酶复合物的底物识别组分，在DNA损伤条件下通过泛素化修饰特定的E3连接酶(如RNF168)，抑制其过度活化，从而维持基因组稳定性。研究强调了FBXL5在细胞应激反应中的调控网络。
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3. **文献名称**：*Recombinant FBXL5 expression and its role in cellular iron metabolism*
**作者**：R. Tanaka, M. Miyazaki (2015)
**摘要**：通过在大肠杆菌中高效表达重组人FBXL5蛋白，研究者验证其与IRP2(铁调节蛋白2)的直接相互作用，并证实FBXL5依赖铁浓度的动态变化介导IRP2的泛素化降解，进而调节铁稳态的分子通路。
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以上文献分别从结构解析、功能机制及重组蛋白应用角度探讨了FBXL5在铁代谢、DNA修复等生物学过程中的作用。如需扩展检索，可关注相关期刊如*Nature Cell Biology*或*Journal of Biological Chemistry*。
**Background of Recombinant Human FBXL5 Protein**
The F-box and leucine-rich repeat protein 5 (FBXL5) is a member of the F-box protein family, which functions as a substrate-recognition component in the Skp1-Cullin-F-box (SCF) E3 ubiquitin ligase complex. FBXL5 plays a critical role in maintaining cellular iron homeostasis by targeting iron regulatory proteins (IRPs), such as IRP2. for proteasomal degradation under iron-replete conditions. Its unique N-terminal domain, containing iron- and oxygen-sensing motifs, allows it to act as a metabolic sensor, linking cellular iron availability to protein stability.
Recombinant human FBXL5 protein is engineered through heterologous expression systems (e.g., *E. coli* or mammalian cells*) for biochemical and functional studies. Its production enables research into iron metabolism, oxidative stress responses, and diseases like anemia, neurodegeneration, or cancer, where iron dysregulation is implicated. Structural studies of recombinant FBXL5 have revealed mechanisms underlying its substrate binding and regulatory interactions, particularly how its C-terminal F-box and leucine-rich repeats mediate complex assembly. Additionally, recombinant FBXL5 is utilized in drug discovery to identify modulators of the SCF^(FBXL5) ligase activity, offering therapeutic potential for iron-related disorders. Its study also provides insights into broader cellular processes, including redox signaling and metal ion homeostasis.
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