| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FBXO11 |
| Uniprot No | Q86XK2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-927aa |
| 氨基酸序列 | MNSVRAANRRPRRVSRPRPVQQQQQQPPQQPPPQPPQQQPPQQQPPPPPQQQQQQQPPPPPPPPPPLPQERNNVGERDDDVPADMVAEESGPGAQNSPYQLRRKTLLPKRTACPTKNSMEGASTSTTENFGHRAKRARVSGKSQDLSAAPAEQYLQEKLPDEVVLKIFSYLLEQDLCRAACVCKRFSELANDPILWKRLYMEVFEYTRPMMHPEPGKFYQINPEEYEHPNPWKESFQQLYKGAHVKPGFAEHFYSNPARYKGRENMLYYDTIEDALGGVQEAHFDGLIFVHSGIYTDEWIYIESPITMIGAAPGKVADKVIIENTRDSTFVFMEGSEDAYVGYMTIRFNPDDKSAQHHNAHHCLEITVNCSPIIDHCIIRSTCTVGSAVCVSGQGACPTIKHCNISDCENVGLYITDHAQGIYEDNEISNNALAGIWVKNHGNPIIRRNHIHHGRDVGVFTFDHGMGYFESCNIHRNRIAGFEVKAYANPTVVRCEIHHGQTGGIYVHEKGRGQFIENKIYANNFAGVWITSNSDPTIRGNSIFNGNQGGVYIFGDGRGLIEGNDIYGNALAGIQIRTNSCPIVRHNKIHDGQHGGIYVHEKGQGVIEENEVYSNTLAGVWVTTGSTPVLRRNRIHSGKQVGVYFYDNGHGVLEDNDIYNHMYSGVQIRTGSNPKIRRNKIWGGQNGGILVYNSGLGCIEDNEIFDNAMAGVWIKTDSNPTLRRNKIHDGRDGGICIFNGGRGLLEENDIFRNAQAGVLISTNSHPILRKNRIFDGFAAGIEITNHATATLEGNQIFNNRFGGLFLASGVNVTMKDNKIMNNQDAIEKAVSRGQCLYKISSYTSYPMHDFYRCHTCNTTDRNAICVNCIKKCHQGHDVEFIRHDRFFCDCGAGTLSNPCTLAGEPTHDTDTLYDSAPPIESNTLQHN |
| 分子量 | 103.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条关于重组人FBXO11蛋白的模拟参考文献(内容基于研究领域常见方向，非真实文献数据)：
1. **"FBXO11 triggers proteasomal degradation of BCL6 and is disrupted in diffuse large B-cell lymphomas"**
- **作者**: Duan Y. et al.
- **摘要**: 研究揭示FBXO11作为SCF泛素连接酶复合体的底物识别组分，通过泛素化降解转录抑制因子BCL6.重组人FBXO11蛋白实验证明其在调控B细胞分化中的关键作用，FBXO11基因缺失与淋巴瘤发生相关。
2. **"Structural insights into the substrate recognition of FBXO11 ubiquitin ligase"**
- **作者**: Chen L. & Wang H.
- **摘要**: 通过X射线晶体学解析重组人FBXO11蛋白与底物结合结构域的三维结构，阐明其特异性识别磷酸化底物的分子机制，为开发靶向FBXO11的小分子抑制剂奠定基础。
3. **"FBXO11 deletion in melanoma enhances PD-L1 expression and promotes immune evasion"**
- **作者**: Zhang R. et al.
- **摘要**: 利用重组FBXO11蛋白进行体外泛素化实验，证实其通过降解STAT3蛋白负向调控PD-L1表达。黑色素瘤中FBXO11的缺失导致免疫检查点分子异常上调，与肿瘤免疫逃逸相关。
(注：以上文献为假设性内容，实际引用需查询PubMed等数据库获取真实文献。)
FBXO11 (F-box protein 11) is a member of the F-box protein family, which functions as a substrate-recognition component of the SCF (SKP1-CUL1-F-box) E3 ubiquitin ligase complex. This complex mediates the ubiquitination and subsequent proteasomal degradation of target proteins, playing a critical role in regulating cellular processes such as the cell cycle, DNA damage response, and chromatin remodeling. The human FBXO11 gene encodes a protein containing an N-terminal F-box domain for binding SKP1 and a C-terminal region with conserved motifs, including a LRR (leucine-rich repeat) domain, which participates in substrate interaction.
Recombinant human FBXO11 protein is produced using expression systems like *E. coli* or mammalian cell cultures, enabling biochemical and functional studies. Research has linked FBXO11 to tumor suppression, as its inactivation or mutation is associated with B-cell lymphoma, nasopharyngeal carcinoma, and developmental disorders. FBXO11 targets substrates such as BCL6 (a transcriptional repressor in lymphocytes) and histone H2AK119ub, highlighting its role in epigenetic regulation and oncogenesis. Studies using recombinant FBXO11 have elucidated its structure-function relationships, substrate specificity, and interaction networks, providing insights into therapeutic strategies for related diseases. Its dynamic regulation in stress responses and genomic stability underscores its biomedical relevance.
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