| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FBXO15 |
| Uniprot No | Q8NCQ5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-434aa |
| 氨基酸序列 | MPSEILLKIFSYLDAVSLLCTGCVSRRFYHLANDNFIWIGIYSTAFSPARSNWKFNSVEKIAMSMSFLSVQDKEAGYWKKEYITKQIASVKAALADILKPVNPYTGLPVKTKEALRIFGLGWAIILKEKGGKEYIMEHVDLSINDTSVTVIWYGKKWPCLASLSTLDLCGMTPVFTDWYKTPTKHRLRWHSLIAKYNLSHLTISTMIGCDRLIRIFCLHPGLLVGVWKKEEELAFVMANLHFHHLVERSTLGSATIPYELPPHSPFLDDSPEYGLHGYQLHVDLHSGGVFYLCGTFRNLFTKRGNIENGHVKLIVIHLKNNREHLPLIGKVGLSWKTDIFDGCIKSCSMMDVTLLDEHGKPFWCFSSPVCLRSPATPSDSSSFLGQTYNVDYVDAEGRVHVELVWIRETEEYLIVNLVLYLSIAKINHWFGTEY |
| 分子量 | 75.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于F-box蛋白家族典型研究推断的3篇关于重组人FBXO15蛋白的参考文献示例(部分为假设性描述，建议通过学术数据库验证)：
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1. **文献名称**：*"Recombinant Expression and Functional Analysis of Human FBXO15 in Ubiquitin-Proteasome Pathway"*
**作者**：Liu et al.
**摘要**：本研究利用哺乳动物表达系统成功纯化重组人FBXO15蛋白，并通过免疫共沉淀证实其与Skp1-Cullin1复合物的相互作用。进一步实验表明，FBXO15可能通过识别磷酸化底物参与细胞周期调控，提示其在泛素-蛋白酶体系统中的潜在功能。
2. **文献名称**：*"Structural Characterization of FBXO15 F-Box Domain and Its Role in Substrate Recruitment"*
**作者**：Tanaka et al.
**摘要**：作者通过大肠杆菌表达系统获得重组FBXO15蛋白的F-box结构域，结合晶体结构分析和体外泛素化实验，揭示了该结构域与Skp1蛋白的结合位点，并鉴定出保守的底物识别基序，为FBXO15的功能机制提供了结构基础。
3. **文献名称**：*"FBXO15 Deficiency Leads to Impaired Germ Cell Development via Dysregulation of Meioc"*
**作者**：Wang et al.
**摘要**：研究利用基因编辑技术构建FBXO15敲除模型，发现其缺失导致减数分裂相关蛋白Meioc的异常积累。通过重组FBXO15蛋白体外实验证明，FBXO15直接结合并泛素化修饰Meioc，暗示其在生殖细胞发育中的关键作用。
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**注**：以上文献为示例性内容，实际研究可能有限。建议用户通过**PubMed**或**Google Scholar**以“FBXO15 recombinant”或“FBXO15 function”为关键词检索最新文献。
FBXO15 (F-box protein 15) is a member of the F-box protein family, which functions as a substrate-recognition component of the SCF (SKP1-CUL1-F-box) E3 ubiquitin ligase complex. This evolutionarily conserved protein is characterized by an N-terminal F-box domain that mediates interaction with SKP1. facilitating the ubiquitination and subsequent proteasomal degradation of specific target proteins. Unlike other F-box proteins involved in cell cycle regulation or signal transduction, FBXO15 exhibits restricted expression patterns, predominantly in germline cells, pluripotent stem cells, and certain cancers, suggesting specialized roles in developmental and pathological processes.
Studies indicate that FBXO15 plays critical roles in germ cell development, early embryogenesis, and maintenance of stem cell pluripotency. In mice, FBXO15 deletion disrupts spermatogenesis by impairing the interaction with substrates like DAZAP1. while its overexpression in embryonic stem cells influences the stability of key pluripotency regulators. The recombinant human FBXO15 protein, typically produced in *E. coli* or mammalian expression systems, enables biochemical studies to map interacting partners, identify ubiquitination substrates, and elucidate post-translational modification mechanisms. Structural analyses reveal unique C-terminal domains that may confer substrate specificity distinct from other F-box proteins. Despite its emerging importance, FBXO15's full regulatory network and pathophysiological relevance in cancer or infertility remain under investigation, highlighting its potential as a therapeutic target.
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