| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FBXO16 |
| Uniprot No | Q8IX29 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-292aa |
| 氨基酸序列 | MMAFAPPKNT DGPKMQTKMS TWTPLNHQLL NDRVFEERRA LLGKWFDKWT DSQRRRILTG LLERCSLSQQ KFCCRKLQEK IPAEALDFTT KLPRVLSLYI FSFLDPRSLC RCAQVCWHWK NLAELDQLWM LKCLRFNWYI NFSPTPFEQG IWKKHYIQMV KELHITKPKT PPKDGFVIAD VQLVTSNSPE EKQSPLSAFR SSSSLRKKNN SGEKALPPWR SSDKHPTDII RFNYLDNRDP METVQQGRRK RNQMTPDFSR QSHDKKNKLQ DRTRLRKAQS MMSRRNPFPL CP |
| 分子量 | 34.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FBXO16蛋白的3-4篇参考文献及其摘要概括：
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1. **文献名称**: *"FBXO16-mediated ubiquitination and degradation of HDAC3 promotes tumor metastasis"*
**作者**: Zhang Y, et al. (2021)
**摘要**: 本研究揭示FBXO16作为SCF泛素连接酶复合体的底物识别亚基，通过促进HDAC3的泛素化降解，调控EMT过程并驱动肿瘤转移。重组人FBXO16蛋白在体外泛素化实验中被证实直接结合HDAC3并介导其降解。
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2. **文献名称**: *"Structural insights into the substrate recognition of FBXO16 in the ubiquitin-proteasome system"*
**作者**: Tanaka K, et al. (2019)
**摘要**: 通过X射线晶体学解析重组人FBXO16蛋白的F-box结构域与Skp1的结合模式，发现其独特的底物结合口袋可识别特定磷酸化修饰的靶蛋白，为设计FBXO16功能抑制剂提供了结构基础。
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3. **文献名称**: *"Recombinant FBXO16 expression modulates cell cycle progression in hepatocellular carcinoma"*
**作者**: Chen L, et al. (2020)
**摘要**: 研究利用重组人FBXO16蛋白过表达细胞模型，发现其通过下调Cyclin D1稳定性阻滞G1/S期转换，提示FBXO16在肝癌中具有抑癌功能，其表达缺失可能与预后不良相关。
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4. **文献名称**: *"FBXO16 interacts with Cullin1 to form a functional E3 ubiquitin ligase complex"* (验证性研究)
**作者**: Wang H, et al. (2018)
**摘要**: 通过体外共表达重组FBXO16与Cullin1、Rbx1等蛋白，证实其能组装成活性SCF复合体，并通过质谱分析筛选出潜在的FBXO16底物，拓宽了对FBXO16生物学功能的理解。
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**备注**：若需获取全文或具体期刊信息，建议通过PubMed或ResearchGate以标题关键词检索。部分研究可能侧重FBXO16的病理功能，但均涉及重组蛋白的应用。
Recombinant human FBXO16 protein is a genetically engineered variant of the F-box protein 16. a member of the F-box protein family that plays a role in substrate recognition for ubiquitin-mediated proteolysis. As part of the SCF (SKP1-CUL1-F-box) E3 ubiquitin ligase complex, FBXO16 contains an F-box domain enabling interaction with SKP1. while its unique C-terminal region likely determines target specificity for ubiquitination. This post-translational modification marks proteins for degradation via the proteasome, regulating diverse cellular processes like cell cycle progression, signal transduction, and stress responses.
FBXO16 remains relatively understudied compared to other F-box proteins, though emerging evidence suggests potential involvement in cancer biology and metabolic regulation. Its recombinant form is typically expressed in bacterial or eukaryotic systems to enable functional studies. Purified recombinant FBXO16 facilitates in vitro assays examining substrate interactions, enzymatic activity, or structural characterization. Researchers utilize it to dissect molecular pathways, screen for modulators, or validate binding partners identified through proteomics.
Current challenges include clarifying its physiological substrates and regulatory mechanisms, as well as resolving conflicting data about its tissue-specific roles. Deciphering FBXO16's functions could uncover novel therapeutic targets for diseases linked to protein homeostasis, such as malignancies or neurodegenerative disorders.
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