| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FBXO43 |
| Uniprot No | Q4G163 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-708aa |
| 氨基酸序列 | MSFKDKDERI SCLEAYVTLT SKSSRFTDET EILKMSQRHS GQAGTEAGNG ADSPPIVNSK YSTFRDFCST SSFQDSGYNE LKSCSFDNID KEYLGKKEKG PTLLYEHPET SGLGLTHPLE SPTQKKKCIL PRKEKDKTPE LCETPKISGK KCLPRRRLNV SFALLKGDFE SQNSSLESSI SQVINLEKNI PSSASGFSRA NNFSPLVTST LKTEEVTSCS QKLRLNFSQQ KTSTIDDSKD DCSLFEVECI SPIQGNNFKD SITHDFSDSS LCINDENACP ELLGSSVSGT TCGTDEDIFV TPISNLVANI RFNASQILSP SPEVRGSIST PEDSGFNSLS LEKSEDSLSD QEGSFQELLQ KHKGTPKVGD TIRKTRHLGR SRRLSTLREQ SSQSETEEEK QIVHPDSEKR AAAASAISEG QLSSDESGDL TFSLKNLSKT PALQLVHELF MKSKRKRLQE NSGHEFLEQG DGEKIAVLQC ILAGLIGKKM GIEKLDILTE LKYRNLKHIL AMVLESLTAE SLCSVWKVSR NWREIVVQDK NANRRRKFYI TQLKTDSEGA VLNVEDAATR LQLLNRSALR SVQAQARIPG SQREQGSTLS PWGEVLTPLA SSSVTHLSSK QEEYVKVAKT LFTDEALKPC PRCQSPAKYQ PYKKRGLCSR TACGFDFCVL CLCAYHGSEE CSRGAAKPRN RKDALPGSAQ SKRNLKRL |
| 分子量 | 78.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FBXO43蛋白的3篇示例参考文献(注：文献为模拟示例，具体研究请以实际出版物为准)：
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1. **文献名称**：*FBXO43 regulates meiotic recombination in human germ cells*
**作者**：Smith A, et al.
**摘要**：本研究首次报道了重组人FBXO43蛋白在减数分裂中的功能，证实其通过SCF泛素化复合体调控同源染色体配对和交叉形成，敲除FBXO43导致生殖细胞分裂异常。
2. **文献名称**：*Structural and functional analysis of the F-box domain in recombinant human FBXO43*
**作者**：Zhang L, et al.
**摘要**：通过体外重组表达FBXO43蛋白，解析其F-box结构域与Skp1的相互作用机制，揭示特定氨基酸突变对底物识别及泛素化活性的影响，为癌症靶向治疗提供依据。
3. **文献名称**：*FBXO43 overexpression promotes gastric cancer progression via ubiquitin-mediated degradation of p21*
**作者**：Wang Y, et al.
**摘要**：研究表明，重组FBXO43蛋白在胃癌细胞中异常高表达，通过降解细胞周期抑制因子p21加速肿瘤增殖;抑制FBXO43可激活p53通路并诱导癌细胞凋亡。
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如需真实文献，建议通过PubMed或Web of Science搜索“FBXO43 recombinant human”或“FBXO43 function”获取近年研究成果。
**Background of Recombinant Human FBXO43 Protein**
The F-box protein 43 (FBXO43), also known as FBX43 or NYD-SP15. is a member of the F-box protein family characterized by the conserved F-box domain, which facilitates interactions with Skp1 and Cul1 in the SCF (Skp1-Cul1-F-box) E3 ubiquitin ligase complex. This complex plays a pivotal role in ubiquitin-mediated proteolysis, a critical regulatory mechanism for protein turnover, cell cycle progression, and signal transduction. FBXO43 is notably implicated in meiosis and spermatogenesis, with studies highlighting its involvement in male germ cell development, particularly during the transition from diploid to haploid states.
Recombinant human FBXO43 protein is engineered in vitro using expression systems (e.g., *E. coli* or mammalian cells) to produce a purified, functional form for mechanistic studies. Its structural features, including the F-box domain and substrate-binding regions, enable investigations into substrate recognition, protein-protein interactions, and ubiquitination pathways. Dysregulation of FBXO43 has been linked to infertility and reproductive disorders, underscoring its biomedical relevance. Current research leverages recombinant FBXO43 to explore its roles in gametogenesis, cancer (via cell cycle modulation), and potential therapeutic targeting. This tool aids in deciphering molecular mechanisms underlying fertility and disease, offering insights for diagnostics and intervention strategies.
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