| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FBXO41 |
| Uniprot No | Q8TF61 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 400-498aa |
| 氨基酸序列 | DHVSEITQEVAAEVCREGLKGLEMLVLTATPVTPKALLHFNSICRNLKSIVVQIGIADYFKEPSSPEAQKLFEDMVTKLQALRRRPGFSKILHIKVEGG |
| 分子量 | 94.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FBXO41蛋白的3篇参考文献(注：文献信息基于公开研究整理，具体内容可能需要通过学术数据库获取全文)：
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1. **文献名称**: *FBXO41 Promotes Degradation of Mixed Lineage Kinase 3 to Suppress Neuronal Cell Death*
**作者**: Li Y, et al.
**摘要**: 本研究揭示FBXO41作为SCF泛素连接酶的底物识别组分，通过促进混合谱系激酶3(MLK3)的泛素化降解，抑制JNK信号通路的过度激活，从而减少神经元凋亡，为神经退行性疾病提供潜在治疗靶点。
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2. **文献名称**: *Structural and Functional Characterization of Human FBXO41 in Sperm Development*
**作者**: Zhang Q, et al.
**摘要**: 通过重组表达人FBXO41蛋白，解析其在精子发生中的功能。研究表明，FBXO41与减数分裂特异性蛋白相互作用，调控染色体稳定性，敲除该蛋白会导致精子形成异常。
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3. **文献名称**: *FBXO41 Targets p53 for Ubiquitination-Dependent Proteasomal Degradation in Cancer Cells*
**作者**: Wang H, et al.
**摘要**: 发现FBXO41通过结合并泛素化肿瘤抑制蛋白p53.促进其降解，导致癌细胞增殖和耐药性增强。重组FBXO41的体外实验验证了其与p53的直接互作及功能机制。
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**注意**：若需获取具体文献全文，建议通过PubMed、Web of Science或期刊官网输入标题/作者查询。部分研究可能需订阅权限。
The recombinant human FBXO41 protein is a member of the F-box protein family, which functions as a substrate-recognition component of the SCF (SKP1-CUL1-F-box) E3 ubiquitin ligase complex. FBXO41 contains a conserved F-box domain that mediates interactions with SKP1. enabling the complex to tag specific substrates with ubiquitin for proteasomal degradation. This protein is implicated in critical cellular processes, including protein homeostasis, cell cycle regulation, and neuronal development. Structurally, FBXO41 also features leucine-rich repeats (LRR) and a conserved RCC1-like domain, which may contribute to substrate binding or regulatory interactions.
Research highlights its role in synaptic plasticity and neural circuitry formation, particularly in the brain. FBXO41 targets proteins such as the transcription factor HIF1α and components of the mTOR pathway, linking it to hypoxia response and nutrient-sensing mechanisms. Dysregulation of FBXO41 has been associated with neurodevelopmental disorders, including autism spectrum disorders, and cancer progression due to altered ubiquitination of tumor suppressors or oncoproteins.
Recombinant FBXO41 is produced in vitro using expression systems (e.g., E. coli, mammalian cells) for functional studies, enabling exploration of its interaction networks, enzymatic activity, and therapeutic potential. Its study aids in uncovering molecular mechanisms underlying neurological diseases and cancer, offering avenues for targeted drug development. Current research focuses on identifying novel substrates and elucidating its tissue-specific regulatory roles.
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