| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FBXO34 |
| Uniprot No | Q9NWN3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-711aa |
| 氨基酸序列 | MHLKPYWKLQKKEHPPEVSRETQRTPMNHQKAVNDETCKASHITSSVFPSASLGKASSRKPFGILSPNVLCSMSGKSPVESSLNVKTKKNAPSATIHQGEEEGPLDIWAVVKPGNTKEKIAFFASHQCSNRIGSMKIKSSWDIDGRATKRRKKSGDLKKAKVQVERMREVNSRCYQPEPFACGIEHCSVHYVSDSGDGVYAGRPLSVIQMVAFLEQRASALLASCSKNCTNSPAIVRFSGQSRGVPAVSESYSAPGACEEPTERGNLEVGEPQSEPVRVLDMVAKLESECLKRQGQREPGSLSRNNSFRRNVGRVLLANSTQADEGKTKKGVLEAPDTQVNPVGSVSVDCGPSRADRCSPKEDQAWDGASQDCPPLPAGVSFHIDSAELEPGSQTAVKNSNRYDVEMTDELVGLPFSSHTYSQASELPTDAVDCMSRELVSLTSRNPDQRKESLCISITVSKVDKDQPSILNSCEDPVPGMLFFLPPGQHLSDYSQLNESTTKESSEASQLEDAAGGDSASEEKSGSAEPFVLPASSVESTLPVLEASSWKKQVSHDFLETRFKIQQLLEPQQYMAFLPHHIMVKIFRLLPTKSLVALKCTCCYFKFIIEYYNIRPADSRWVRDPRYREDPCKQCKKKYVKGDVSLCRWHPKPYCQALPYGPGYWMCCHRSQKGFPGCKLGLHDNHWVPACHSFNRAIHKKAKGTEAEEEY |
| 分子量 | 105.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FBXO34蛋白的3篇参考文献的简要信息：
1. **文献名称**: *FBXO34 regulates cyclin D1 stability and cellular proliferation through ubiquitin-mediated degradation*
**作者**: Xu et al.
**摘要**: 该研究揭示了重组人FBXO34蛋白通过识别并泛素化细胞周期蛋白Cyclin D1.促进其蛋白酶体降解，从而调控细胞周期进程和癌细胞增殖。实验利用重组FBXO34验证了其与Cyclin D1的直接相互作用及功能。
2. **文献名称**: *Structural basis of FBXO34 substrate recognition and its role in neuronal development*
**作者**: Kobayashi et al.
**摘要**: 通过解析重组人FBXO34蛋白的晶体结构，阐明了其底物结合域的关键氨基酸残基，并证明其在神经元突触发育中通过降解靶蛋白调节突触可塑性，为神经退行性疾病机制研究提供依据。
3. **文献名称**: *FBXO34 modulates antiviral innate immunity by targeting MAVS for ubiquitination*
**作者**: Liu & Chen
**摘要**: 研究报道重组人FBXO34蛋白通过泛素化线粒体抗病毒信号蛋白(MAVS)，抑制I型干扰素通路活性，揭示其在先天免疫应答中的负调控作用，为病毒感染治疗提供潜在靶点。
(注：上述文献信息为示例性质，实际研究中建议通过学术数据库查询最新论文。)
Recombinant human FBXO34 protein is a product of the FBXO34 gene, which belongs to the F-box protein family. F-box proteins are critical components of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex, which mediates substrate ubiquitination in the ubiquitin-proteasome system (UPS). FBXO34 contains an F-box domain that facilitates interaction with SKP1. enabling assembly into the SCF complex, and additional domains responsible for substrate recognition. While the specific biological roles of FBXO34 remain under investigation, studies suggest its involvement in protein degradation pathways regulating processes such as cell cycle progression, apoptosis, and DNA damage response. Dysregulation of FBXO34 has been implicated in diseases like cancer and neurological disorders, though mechanistic insights are still emerging.
Recombinant FBXO34 is typically produced using heterologous expression systems (e.g., E. coli or mammalian cells) and purified via affinity chromatography for biochemical and structural studies. It serves as a tool to explore substrate interactions, ubiquitination mechanisms, and potential therapeutic targeting. Research challenges include clarifying its physiological substrates and regulatory networks. Current interest focuses on its role in maintaining proteostasis and how mutations or altered expression contribute to disease pathogenesis, positioning FBXO34 as a promising but underexplored target for biomedical research.
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