| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FBXO27 |
| Uniprot No | Q8NI29 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-283aa |
| 氨基酸序列 | MGASVSRGRAARVPAPEPEPEEALDLSQLPPELLLVVLSHVPPRTLLGRCRQVCRGWRALVDGQALWLLILARDHGATGRALLHLARSCQSPARNARPCPLGRFCARRPIGRNLIRNPCGQEGLRKWMVQHGGDGWVVEENRTTVPGAPSQTCFVTSFSWCCKKQVLDLEEEGLWPELLDSGRIEICVSDWWGARHDSGCMYRLLVQLLDANQTVLDKFSAVPDPIPQWNNNACLHVTHVFSNIKMGVRFVSFEHRGQDTQFWAGHYGARVTNSSVIVRVRLS |
| 分子量 | 58 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FBXO27蛋白的3篇假设性文献示例(内容基于领域内常见研究方向综合虚构，仅供参考)：
---
1. **文献名称**：*Structural Insights into Human FBXO27 and Its Interaction with SKP1-CUL1 Complex*
**作者**：Chen, X., et al.
**摘要**：通过X射线晶体学解析了FBXO27蛋白的F-box结构域与SKP1的结合模式，揭示了其参与SCF泛素连接酶复合体组装的关键位点。研究还预测了FBXO27可能通过C端未知结构域识别特异性底物。
2. **文献名称**：*FBXO27 Regulates Synaptic Protein Degradation and Neuronal Morphogenesis*
**作者**：Zhang, Y., et al.
**摘要**：利用CRISPR敲低FBXO27基因，发现其缺失导致神经元突触中特定跨膜蛋白(如DPP6)异常积累，并引发突触功能紊乱，表明FBXO27通过泛素-蛋白酶体系统维持突触蛋白质稳态。
3. **文献名称**：*Dysregulation of FBXO27 in Alzheimer's Disease: A Link to Tau Pathology*
**作者**：Wang, L., et al.
**摘要**：发现FBXO27在阿尔茨海默病患者脑组织中表达降低，且其过表达可促进磷酸化Tau蛋白的泛素化降解，提示FBXO27功能异常可能参与神经纤维缠结形成机制。
---
(注：以上文献为示例，实际研究需查询真实数据库如PubMed。)
Recombinant human FBXO27 protein is a genetically engineered form of the F-box protein 27. a member of the F-box protein family that serves as a substrate-recognition component in the SCF (SKP1-CUL1-F-box) E3 ubiquitin ligase complex. This complex regulates ubiquitination, a post-translational modification that tags proteins for degradation via the proteasome. FBXO27 is characterized by its conserved F-box domain, which interacts with SKP1 to anchor the protein within the SCF machinery. Unlike some F-box proteins with well-defined substrates, FBXO27 remains less understood, though studies suggest roles in neuronal development, vesicle trafficking, and immune regulation. Research highlights its potential involvement in neurological disorders, cancer, and viral infections, possibly through interactions with cellular targets like SNARE proteins or viral glycoproteins. Recombinant FBXO27 is typically expressed in prokaryotic (e.g., E. coli) or eukaryotic systems to enable functional studies, structural analysis (e.g., crystallography), or interaction mapping. Its production allows researchers to investigate mechanisms of ubiquitination, protein turnover dynamics, and disease-associated mutations. Ongoing efforts aim to clarify its physiological substrates, regulatory pathways, and therapeutic relevance, particularly in neurodegeneration and intracellular pathogen responses.
×
