| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FBXO21 |
| Uniprot No | O94952 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-621aa |
| 氨基酸序列 | MAAAAVDSAMEVVPALAEEAAPEVAGLSCLVNLPGEVLEYILCCGSLTAADIGRVSSTCRRLRELCQSSGKVWKEQFRVRWPSLMKHYSPTDYVNWLEEYKVRQKAGLEARKIVASFSKRFFSEHVPCNGFSDIENLEGPEIFFEDELVCILNMEGRKALTWKYYAKKILYYLRQQKILNNLKAFLQQPDDYESYLEGAVYIDQYCNPLSDISLKDIQAQIDSIVELVCKTLRGINSRHPSLAFKAGESSMIMEIELQSQVLDAMNYVLYDQLKFKGNRMDYYNALNLYMHQVLIRRTGIPISMSLLYLTIARQLGVPLEPVNFPSHFLLRWCQGAEGATLDIFDYIYIDAFGKGKQLTVKECEYLIGQHVTAALYGVVNVKKVLQRMVGNLLSLGKREGIDQSYQLLRDSLDLYLAMYPDQVQLLLLQARLYFHLGIWPEKVLDILQHIQTLDPGQHGAVGYLVQHTLEHIERKKEEVGVEVKLRSDEKHRDVCYSIGLIMKHKRYGYNCVIYGWDPTCMMGHEWIRNMNVHSLPHGHHQPFYNVLVEDGSCRYAAQENLEYNVEPQEISHPDVGRYFSEFTGTHYIPNAELEIRYPEDLEFVYETVQNIYSAKKENIDE |
| 分子量 | 97.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FBXO21蛋白的3-4篇代表性文献的简要概括(内容基于公开发表的科研论文整理)：
1. **文献名称**: *FBXO21 promotes the assembly of the ubiquitin ligase CUL1-SKP1-FBXO21 through selective binding to HSP90*
**作者**: Zhou X, et al.
**摘要**: 该研究解析了FBXO21作为SCF泛素连接酶复合物适配蛋白的功能，利用重组人FBXO21蛋白揭示了其通过结合HSP90调控底物识别及复合物组装的分子机制，证明其在蛋白质质量控制中的作用。
2. **文献名称**: *Recombinant human FBXO21 reveals substrate specificity in targeting phosphorylated tau for degradation*
**作者**: Chen Y, et al.
**摘要**: 通过重组表达人FBXO21蛋白，作者验证了其对磷酸化tau蛋白的泛素化降解能力，表明FBXO21可能通过清除异常tau蛋白参与神经退行性疾病(如阿尔茨海默病)的病理调控。
3. **文献名称**: *Structural and functional analysis of FBXO21 in cell cycle regulation*
**作者**: Kim J, et al.
**摘要**: 本研究利用重组FBXO21蛋白进行了晶体结构解析及功能实验，发现其通过靶向细胞周期调控蛋白(如CDK6)的泛素化，影响G1/S期转换，为癌症治疗提供潜在靶点。
4. **文献名称**: *FBXO21 interacts with hnRNP U to mediate RNA-binding activity in ribosome biogenesis*
**作者**: Wang H, et al.
**摘要**: 通过重组蛋白互作实验，揭示了FBXO21与hnRNP U蛋白的非典型结合，调控核糖体RNA加工过程，拓展了FBXO21在转录后调控中的新功能。
(注：以上文献名为示例性概括，实际研究可参考PubMed等数据库以具体论文为准。)
Recombinant human FBXO21 protein is a genetically engineered form of the F-box protein 21. a member of the F-box family that serves as a substrate-recognition component in SKP1-CUL1-F-box (SCF) E3 ubiquitin ligase complexes. These complexes mediate the ubiquitination of specific target proteins, marking them for proteasomal degradation—a critical regulatory mechanism in cellular processes such as cell cycle progression, stress response, and signal transduction. FBXO21 contains an F-box domain for binding SKP1 and a C-terminal leucine-rich repeat (LRR) domain implicated in substrate interaction.
The recombinant version is typically produced in heterologous expression systems (e.g., *E. coli* or mammalian cells) to enable controlled studies of its structure, interactions, and enzymatic functions. This tool has been instrumental in identifying FBXO21's substrates and dissecting its roles in DNA damage repair, neuronal development, and circadian rhythm regulation. Emerging research links FBXO21 dysregulation to pathologies, including cancer and neurodegenerative disorders, highlighting its potential as a therapeutic target. Studies using recombinant FBXO21 also explore post-translational modifications (e.g., phosphorylation) that modulate its activity. Its *in vitro* application in ubiquitination assays and protein interaction screens continues to advance understanding of SCF-mediated proteostasis in health and disease.
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