| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FBXO17 |
| Uniprot No | Q96EF6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-287aa |
| 氨基酸序列 | MKQGLWLLEMGARLSRRRLPADPSLALDALPPELLVQVLSHVPPRSLVTRCRPVCRAWRDIVDGPTVWLLQLARDRSAEGRALYAVAQRCLPSNEDKEEFPLCALARYCLRAPFGRNLIFNSCGEQGFRGWEVEHGGNGWAIEKNLTPVPGAPSQTCFVTSFEWCSKRQLVDLVMEGVWQELLDSAQIEICVADWWGARENCGCVYQLRVRLLDVYEKEVVKFSASPDPVLQWTERGCRQVSHVFTNFGKGIRYVSFEQYGRDVSSWVGHYGALVTHSSVRVRIRLS |
| 分子量 | 59 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FBXO17蛋白的示例参考文献(内容为模拟虚构，仅供参考)：
1. **《FBXO17 mediates substrate recognition in ubiquitin-dependent degradation pathways》**
- 作者：Chen L, et al. (2022)
- 摘要：本研究利用重组人FBXO17蛋白解析其与E3泛素连接酶复合体(SCF复合体)的相互作用，揭示了FBXO17通过C端结构域识别磷酸化底物，并调控细胞周期蛋白的泛素化降解。
2. **《Functional characterization of recombinant human FBXO17 in spermatogenesis》**
- 作者：Santiyanont R, et al. (2019)
- 摘要：通过重组表达FBXO17蛋白，发现其在哺乳动物精子形成过程中与减数分裂调控相关，可能通过靶向特定蛋白的降解维持生殖细胞基因组稳定性。
3. **《Structural insights into the F-box protein FBXO17 and its interaction with SKP1》**
- 作者：Zhang Y, et al. (2021)
- 摘要：利用X射线晶体学分析重组人FBXO17蛋白的结构，明确了其F-box结构域与SKP1的结合模式，为开发SCF复合体抑制剂提供了理论基础。
4. **《FBXO17 promotes cancer metastasis via stabilizing oncogenic transcription factors》**
- 作者：Kim S, et al. (2020)
- 摘要：研究发现重组FBXO17蛋白可通过非典型的泛素化通路稳定促癌因子，如c-MYC，从而增强肿瘤细胞的侵袭和转移能力。
**注**：以上文献为模拟示例，实际研究中建议通过PubMed或Web of Science以“FBXO17”“SCF complex”“ubiquitination”等关键词检索最新文献。
**Background of Recombinant Human FBXO17 Protein**
FBXO17 (F-box protein 17) is a member of the F-box protein family, characterized by a conserved F-box domain that mediates interactions with SKP1. a core component of the SCF (SKP1-Cullin-F-box) ubiquitin ligase complex. SCF complexes regulate protein degradation via the ubiquitin-proteasome system, impacting critical cellular processes like cell cycle progression, signal transduction, and stress responses. FBXO17 is postulated to act as a substrate-recognition subunit, targeting specific proteins for ubiquitination and subsequent degradation, though its precise physiological substrates and regulatory mechanisms remain poorly characterized.
Structurally, FBXO17 contains additional domains beyond the F-box motif, including a C-terminal region with predicted protein-binding motifs, suggesting roles in diverse molecular interactions. Studies link FBXO17 to cellular functions such as DNA damage repair, apoptosis, and transcriptional regulation. Dysregulation of FBXO17 has been implicated in cancer, neurodegenerative disorders, and immune responses, though evidence is preliminary and context-dependent.
Recombinant human FBXO17 protein is typically produced in *E. coli* or mammalian expression systems, enabling biochemical and functional studies. Its recombinant form facilitates investigations into substrate binding, enzymatic activity, and interactions within the ubiquitination pathway. Despite emerging interest, FBXO17 remains understudied compared to other F-box proteins, necessitating further research to unravel its biological significance and therapeutic potential in disease pathways.
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